Up-regulate of the proportion of CD4+CD25+ regulatory T cells by lymphoma cell EL-4 supernatants / 肿瘤研究与临床
Cancer Research and Clinic
; (6): 239-241, 2009.
Article
em Zh
| WPRIM
| ID: wpr-381072
Biblioteca responsável:
WPRO
ABSTRACT
Objective To explore if the tumor cell can up-regulate the proliferation of CD4+CD25+ Treg cells. Methods Lymphoma cell EL-4 supernatant was combined with the normal mise spleen lymphocyte. After cultured 72 hours, CD4+CD25+ subset were analyzed by flow cytometry and the gene expression level of the specific marker Foxp3 were tested by RT-PCR. The experiment repeated three times. Results The supematant derived from EL-4 can increase the proportion of CD4+CD25+ Treg cells in normal mouse spleen lymphocytes than that of the control group. Moreover, when added the CD3 McAb with EL-4 supematant together, the number of CD4+CD25+ Treg cells increased. The level of corresponding Foxp3 mRNA also increased. Conclusion These data suggested that in the supernatant may occur immune regulatory factors which up-regulated the number of CD4+CD25+ Treg cells and indicated that the tumorigenesis can facilitate proliferation of CD4+CD25+ Treg cells.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Cancer Research and Clinic
Ano de publicação:
2009
Tipo de documento:
Article