The Analysis of Vitamin C Concentration in Organs of Gulo-/- Mice Upon Vitamin C Withdrawal
Immune Network
; : 18-26, 2012.
Article
em En
| WPRIM
| ID: wpr-39028
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: Vitamin C is an essential nutrient for maintaining human life. Vitamin C insufficiency in the plasma is closely related with the development of scurvy. However, in vivo kinetics of vitamin C regarding its storage and consumption is still largely unknown. METHODS: We used Gulo-/- mice, which cannot synthesize vitamin C like human. Vitamin C level in plasma and organs from Gulo-/- mice was examined, and it compared with the level of wild-type mice during 5 weeks. RESULTS: The significant weight loss of Gulo-/- mice was shown at 3 weeks after vitamin C withdrawal. However, there was no differences between wild-type and vitamin C-supplemented Gulo-/- mice (3.3 g/L in drinking water). The concentration of vitamin C in plasma and organs was significantly decreased at 1 week after vitamin C withdrawal. Vitamin C is preferentially deposited in adrenal gland, lymph node, lung, and brain. There were no significant changes in the numbers and CD4/CD8 ratio of splenocytes in Gulo-/- mice with vitamin C withdrawal for 4 weeks. And the architecture of spleen in Gulo-/- mice was disrupted at 5 weeks after vitamin C withdrawal. CONCLUSION: The vitamin C level of Gulo-/- mice was considerably decreased from 1 week after vitamin C withdrawal. Vitamin C is preferentially stored in some organs such as brain, adrenal gland and lung.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Plasma
/
Ácido Ascórbico
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Escorbuto
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Baço
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Vitaminas
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Encéfalo
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Cinética
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Redução de Peso
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Glândulas Suprarrenais
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Ingestão de Líquidos
Limite:
Animals
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Humans
Idioma:
En
Revista:
Immune Network
Ano de publicação:
2012
Tipo de documento:
Article