Nano-hydroxyapatite/collagen/poly(L-lactic acid)co-cultured with bone marrow stromal cells for repair of rabbit mandibular defect

Chengyue WANG; Wei WANG; Li ZHANG; Hongjun AI; Fuzhai CUI

ABSTRACT

BACKGROUND:

Selection of proper bone transplantation materials to substitute autogenous bone for treatment of jaw bone defect in patients has become the focus of current researches.

OBJECTIVE:

To observe the ability of tissue engineered bone constructed with nano-hydroxyapatite/collagen/poly(L-lactic acid)(nHAC/PLA)co-cultured with bone marrow stromal cells(BMSCs)in treatment of rabbit mandibular defect.and to make a comparison with autogenous bone and simple nHAC/PLA.DESIGN.TIME AND

SETTING:

A randomized controlled animal experiment was performed at the Animal Laboratory of Jinzhou Central Hospital between March and October 2007.MATERIALSA total of 40 New Zealand rabbits were randomized to 4 groups.with 10 rabbits per groupnHAC/PLA co-cultured with rabbit BMSCs(BMSCs/nHAC/PLA),autogenous bone,nHAC/PLA,and control.

METHODS:

A 15 mm×15 mm full-thickness bone defect was created in rabbit mandible body.In the BMSCs/nHAC/PLA group.bone defect was repaired by nHAC/PLA co-cultured with BMSCs for 14 days in vitro;the bone defect in the autogenous bone and nHAC/PLA groups was treated by autogenous iliac bone and nHAC/PLA.respectively;while the control group received no any treatment.MAIN OUTCOME

MEASURES:

At 1,3,and 6 months after surgery,bone mineral density(BMD)measurement and histological examination were performed to evaluate the repairing effects of bone defect.

RESULTS:

The BMSCs/nHAC/PLA group exhibited the BMD similar to the autogenous bone group(P＞0.05),but it was significantly higher compared to the nHAC/PLA group and the blank control group(P＜0.01).At 6 months after surgery.newly formed bone tissue grew towards large bulk and bridged defected stumps.and the seaffold materials were hardly seen in the BMSCs/nHAC/PLA and autogenous bone groups;simultaneously.the nHAC/PLA group exhibited increased trabecular bone with many fibrous tissues and incompletely degraded seaffold materials.

CONCLUSION:

BMSCs/nHAC/PLA seaffold exhibits the effects similar to autogenous bone.but superior to nHAC/PLA seaffold in the treatment of mandibular defects.