Vitamin C acts indirectly to modulate isotype switching in mouse B cells / 대한해부학회지
Anatomy & Cell Biology
; : 25-35, 2010.
Article
em En
| WPRIM
| ID: wpr-43659
Biblioteca responsável:
WPRO
ABSTRACT
Vitamin C, one of essential micronutrients, has been reported to modulate the humoral immune responses in some mammals. We investigated whether vitamin C might modulate this response in mice by directly affecting B cells. Splenic B cells were isolated and activated by CD40- and B cell receptor-ligation in vitro. The cells were cultured with a pretreatment of vitamin C from 0 to 1 mM of concentrations. Vitamin C slightly increased apoptosis of B cells dose-dependently and behaved as an antioxidant. We found that in vivo administration of vitamin C by intraperitoneal injection affected isotype switching as previously reported: the titer of antigen-specific IgG1 antibody was decreased, while that of IgG2a was unaffected. Somewhat different from those observed in vivo, in vitro exposure to vitamin C slightly decreased isotype switching to IgG1 and increased isotype switching to IgG2a. Pretreatment with vitamin C in the safe range did not affect either proliferation of cultured B cells or the expression of CD80 and CD86 in those cells. Taken together, in vivo results suggest that vitamin C acts to modulate isotype switching in the mouse. However, because of our in vitro results, we suggest that the modulation exerted by vitamin C in vivo is by indirectly affecting B cells, perhaps by directly influencing other immune cells such as dendritic cells.
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Base de dados:
WPRIM
Assunto principal:
Ácido Ascórbico
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Vitaminas
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Células Dendríticas
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Imunoglobulina G
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Linfócitos B
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Espécies Reativas de Oxigênio
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Apoptose
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Switching de Imunoglobulina
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Micronutrientes
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Imunidade Humoral
Limite:
Animals
Idioma:
En
Revista:
Anatomy & Cell Biology
Ano de publicação:
2010
Tipo de documento:
Article