The Change of Bone Metabolism in Ovariectomized Rats : Analyses of MicroCT Scan and Biochemical Markers of Bone Turnover
Journal of Korean Neurosurgical Society
; : 323-327, 2012.
Article
em En
| WPRIM
| ID: wpr-45153
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE: The purpose of this study was to verify the appropriateness of ovariectomized rats as the osteoporosis animal model. METHODS: Twelve female Sprague-Dawley rats underwent a sham operation (the sham group) or bilateral ovariectomy [the ovariectomy (OVX) group]. Eight weeks after operations, serum biochemical markers of bone turnover were analyzed; osteocalcin and alkaline phosphatase, which are sensitive biochemical markers of bone formation, and C-terminal telopeptide fragment of type I collagen C-terminus (CTX), which is a sensitive biochemical marker of bone resorption. Bone histomorphometric parameters and microarchitectural properties of 4th lumbar vertebrae were determined by micro-computed tomographic (CT) scan. RESULTS: The OVX group showed on average 75.4% higher osteocalcin and 72.5% higher CTX levels than the sham group, indicating increased bone turnover. Micro-CT analysis showed significantly lower bone mineral density (BMD) (p=0.005) and cortical BMD (p=0.021) in the OVX group. Furthermore, the OVX group was found to have a significantly lower trabecular bone volume fraction (p=0.002). CONCLUSION: Our results showed that bone turnover was significantly increased and bone mass was significantly decreased 8 weeks after ovariectomy in rats. Thus, we propose that the ovariectomized rat model be considered a reproducible and reliable model of osteoporosis.
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Base de dados:
WPRIM
Assunto principal:
Osteogênese
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Osteoporose
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Reabsorção Óssea
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Ovariectomia
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Biomarcadores
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Densidade Óssea
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Osteocalcina
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Salicilamidas
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Ratos Sprague-Dawley
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Colágeno Tipo I
Limite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Journal of Korean Neurosurgical Society
Ano de publicação:
2012
Tipo de documento:
Article