The development and application of stabilizers for ELISA test kit / 国际检验医学杂志
International Journal of Laboratory Medicine
; (12): 2527-2528, 2014.
Article
em Zh
| WPRIM
| ID: wpr-454357
Biblioteca responsável:
WPRO
ABSTRACT
Objective To develop ELISA kit stabilizer for each component ,with which the shelf life of kit could meet the com-mercial need .Methods A series of stabilizers for microplate ,enzyme-labeled antibody and standards were prepared by the means of experience and orthogonal approach ,through controlled trials ,screening out the best stabilizer formulations .Results A kind of coa-ted stabilizer (mass fraction :bovine serum albumin 0 .5% ,gelatin 0 .25% ,trehalose 5% ,PEG4000 0 .1% ) and a kind of enzyme la-beled antibody stabilizer (mass fraction :Bovine serum albumin 1% ,peptone 1% ,sucrose 10% ,trehalose 5% ,PEG4000 0 .25% ) were screened out .Conclusion The ELISA kits can be stable at 4 ℃ for 1 year after treated with the obtained stabilizers .
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1
Base de dados:
WPRIM
Idioma:
Zh
Revista:
International Journal of Laboratory Medicine
Ano de publicação:
2014
Tipo de documento:
Article