Antioxidant Activity of Xanthium Strumarium L. Extracted F ractions in Lens Epithelial Cells
Journal of the Korean Ophthalmological Society
; : 152-159, 2001.
Artigo
em Coreano
| WPRIM (Pacífico Ocidental)
| ID: wpr-46849
Biblioteca responsável:
WPRO
ABSTRACT
To elucidate the antioxidant effect of Xanthium strumarium L., a fruit of Dokomari or Daekori, which is a family of chrysanthemum on the H2O2-mediated cellualr damage, we examined the effect of Xanthium strumarium L. extraction fractions on survival of human lens epithelia, HLE B-3 cells by using cell culture system. H2O2-mediated cellualr death and its IC5 0, with approximately 100 micrometer H2O2 were determined by using MTT assay. The HLE B-3 cells pretreated with Xanthium strumarium L. extract fractions, were incubated with 100 micrometer H2O2, and in order to assess the cell viability the cultures were incubated with MTT solution. Among Xanthium strumarium L. extract fractions, Xan crude fraction, Xan CHCl3 fraction, and Xan EtAc fraction have antioxidant activity at the concentrations of 500 ng/ml, 1 microgram/ml and 100 ng/ml, respectively. These effects were statistically significant(p<0.05). Also, the fact that only the frations extracted with organic solvents have antioxidant activity suggests that the active components from the extract have hydrophobic property.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Solventes
/
Catarata
/
Sobrevivência Celular
/
Estresse Oxidativo
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Técnicas de Cultura de Células
/
Chrysanthemum
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Xanthium
/
Células Epiteliais
/
Frutas
/
Antioxidantes
Limite:
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Ophthalmological Society
Ano de publicação:
2001
Tipo de documento:
Artigo