A novel cDNA clone related with rat liver regeneration / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1989.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-519266
Biblioteca responsável:
WPRO
ABSTRACT
AIM:
To study a novel gene that probably related with liver regeneration, which was found by representational difference analysis(RDA).METHODS:
cDNA sequence, tissue distribution and functions of the novel gene were studied by slot blot, Northern blot, RT-PCR, cDNA library screening and sequence analyzing.RESULTS:
Two full-length clones were isolated from cDNA library of rat fetal livers and the sequence analysis identified that the positive cDNA encoded 76 amino acids only; Using the cDNA as a probe, the novel gene showed a specific liver distribution, a moment increasing expression in one hour after partial hepatectomy (PH) and high expression in fetal liver or liver tumor by Northern blot; EGF quickly induced its high expression in primary culture rat hepatocytes(FCS free).CONCLUSION:
These results show that the novel gene is an early phase response gene that is closely related to a liver regeneration adjustment. It may encode peptide or has longer sequence at N tip.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
1989
Tipo de documento:
Artigo