Interaction between human augmenter of liver regeneration and Na~+-K~+-ATPase in vitro / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1986.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-530310
Biblioteca responsável:
WPRO
ABSTRACT
AIM:
To check the physical interaction between GST-Na+-K+-ATPase domain and recombinant human augmenter of liver regeneration (rhALR) by GST pull down assay.METHODS:
With PCR and genetic recombinant techniques, the coding region of ? subunit of Na+-K+-ATPase was cloned into expressing plasmid pGEX-4T and identified by endonuclease digestion and sequencing methods. Under the inducing of 0.1 mmol/L IPTG, the fusion protein GST-Na+-K+-ATPase domain was highly expressed by E.coli DH-5?. After hypersound quassating, the GST-Na+-K+-ATPase domain was purified by glutathione agarose beads and the physical interaction with rhALR was checked by GST pull down assay.RESULTS:
Analysis by SDS-PAGE showed the rhALRs of monomer and dimmer in GST-Na+-K+-ATPase domain lane. The Western blotting of the GST-pull down assay showed the same results as well.CONCLUSION:
The Na+-K+-ATPase domain is associated with rhALR specifically in vitro.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
1986
Tipo de documento:
Artigo