Mechanism responsible for inhibitory effect of recombinant rat augmenter of liver regeneration on renal turbular epithelial cell apoptosis by gentamycin / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1986.
Article
em Zh
| WPRIM
| ID: wpr-531829
Biblioteca responsável:
WPRO
ABSTRACT
AIM:To investigate the effects of recombinant rat augmenter of liver regeneration (rrALR) on apoptosis of renal tubular cells (NRK-52E cells) induced by gentamycin sulfate (GM). METHODS: The cultured NRK-52E cells were divided into four groups: normal control cells, cells with GM (GM 1.6 g/L) or GM and rrALR (15 mg/L or 25 mg/L) treatments. The apoptosis of cultured cells were assessed at 24 h, 48 h by AO/EB staining, DNA agarose gel electrophoresis analysis and flow cytometry using Annexin V-FITC and propidium iodide (PI) staining. The protein and mRNA expressions of Bcl-2 and Bax were detected by Western blotting and RT-PCR, respectively. RESULTS: (1) rrALR inhibited NRK-52E cells apoptosis induced by GM (P
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Pathophysiology
Ano de publicação:
1986
Tipo de documento:
Article