Effect of leptin on protecting rat islet ?-cells against lipotoxicity induced by free fatty acid / 中华内分泌代谢杂志
Chinese Journal of Endocrinology and Metabolism
; (12)1986.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-538119
Biblioteca responsável:
WPRO
ABSTRACT
Objective To observe whether leptin protects rat islet ?-cells from lipotoxicity induced by free Taity acid ( FFA). Methods Pancreatic islets were isolated from Wistar rats. Isolated islets were divided into 4 groups; ( 1 ) Control group; (2) Leptin group (murine recombinanl leptin 100?g/L) ; (3) FFA group [0. 25 mmol/L long chain fatty acid (oleate palmitate =2 1 ) ] ; (4) FFA + Leptin group (both leptin and FFA were added to the culture medium). Alter being cultured lor 72 hours, triglyceride ( TG) content in islets, insulin secreted under basal and glucose stimulated conditions, and the mRNA expression of fatty acid oxidase-carnitine palmityl transferase- 1 ( CFT- I ) , fatty acid synthetase-acyl-CoA carboxylase ( ACC) , peroxisome proliferator activated receptors ( PPAR)a and PPARr in islets were examined. Results ( 1 ) TC content in islets was elevated in FFA group as compared with control group ( P
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Endocrinology and Metabolism
Ano de publicação:
1986
Tipo de documento:
Artigo