F-ATPase subunit uncEBF mRNA expression in Streptococcus mutans clinical isolates / 实用口腔医学杂志
Journal of Practical Stomatology
; (6)2000.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-542177
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To investigate the gene expression variety of different genotype of F-ATPase subunit uncEBF in Streptococcus mutans (S.mutans) and to evaluate the relationship among uncEBF gene expression levels, genotypes and the acidurance ability of S.mutans.Methods:
The relative expression quantity of uncEBF gene against the housekeeping gene recA was determined by the two-step method of semi-quantitative RT-PCR in 18 clinical isolates of S.mutans(7 with genotype A uncEBF and 11 with genotyp B,10 with high acidurance and 8 with low). A gel documentation system and QUANTITY ONES software were used to assay the data.Results:
uncEBF mRNA expression level in the isolates with genotype A uncEBF was higher than that in those with genotype B(P
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Practical Stomatology
Ano de publicação:
2000
Tipo de documento:
Artigo