Growth property of rat hair follicle stem cells cultured in serum-free K-SFM in vitro / 第三军医大学学报

ABSTRACT

Objective To study the change of growth property of rat hair follicle stem cells cultured in serum-free K-SFM in vitro. Methods Rat hair follicle bulge cells were isolated and cultured in two different conditions, serum-free K-SFM and DMEM/F12 with 10% fetal calf serum. Growth property, colony-forming efficiency (CFE), and K19 expression were analyzed. Results The CFE and positive expression of K19 were significantly higher in bulge cells cultured in serum-free K-SFM than that of DMEM/F12 with 10% fetal calf serum (P