Effect of recombinant rat augmenter of liver regeneration on proliferation and apoptosis of renal tubular cells / 中华肾脏病杂志
Chinese Journal of Nephrology
; (12)2005.
Article
em Zh
| WPRIM
| ID: wpr-557805
Biblioteca responsável:
WPRO
ABSTRACT
Objective To study the effect of recombinant rat augmenter of liver regeneration (rrALR) on proliferation and apoptosis of renal tubular cells in vitro. Methods The cultured human renal tubular cell line (HK2 cells) was divided into several groups with various concentration of gentamicin(GM) or/and rrALR. The proliferation of HK2 cell was evaluated by 3H-TdR incorporation. The apoptosis of HK2 cells was assessed by AO/EB staining and flow cytometer using Annexin V-FITC and propidium iodide (PI) staining. Results (1 ) rrALR promoted HK2 cell proliferation in a time-dependent and dose-dependent manner. The proliferative effect was significant with the concentration from 25 ng/ml to 50 ug/ml (P
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Nephrology
Ano de publicação:
2005
Tipo de documento:
Article