Relationship of IL-6/STAT3 with liver regeneration of partial grafts after cold preservation in rats / 第三军医大学学报

ABSTRACT

Objective To evaluate the effect of different cold preservation time on liver regeneration after partial liver transplantation in rats,and study the role of IL-6/STAT3.Methods SD rats were divided into 3 groupsgroup Ⅰ underwent liver resection,group Ⅱ received partial liver transplantation after one-hour cold preservation and group Ⅲ received partial liver transplantation after 8-hour cold preservation.Radioimmunoassay was used to measure the levels of TNF-? and IL-6 in liver tissues.The protein expressions of PCNA and STAT3 were assessed by immunohistochemical staining in paraffin sections.The liver regeneration ratio and the expressions of the proliferative cell nuclear antigen(PCNA)and STAT3 were compared among three groups at 1,6,12,24,48,72 and 168 h after operation.Results The remnant liver in group Ⅰ and grafts in group Ⅱ grew rapidly;The regeneration ratio in group Ⅲ was significantly lower than that in group Ⅰ and group Ⅱ during postoperative day 2 to 3(P