Effects of +Gz Exposures on Expression of HSP70 Protein in Rat Hippocampus / 航天医学与医学工程

ABSTRACT

Objective To observe changes of expression level of HSP70 protein in rat hippocampus after exposure to +Gz of different magnitudes and to investigate the machanism of HSP70 protein induced by +Gz exposure.Method One hundred rats were divided into control group,+2 Gz group,+6 Gz group and +10 Gz group.The experimental rats were exposed to +2 Gz,+6 Gz and +10 Gz for 3 min respectively.The rats were killed 6 h,1 d,2 d,4 d,6 d after exposure and brain specimens were made for examination under microscope.The changes of HSP70 protein expression level were determined with immunohistochemical technology.Result The HSP70 protein expression level in the 3 experimental groups were significantly higher than that in control group.It began to rise 6 h after exposure,reached the peak after 1 d,and resumed to normal 6 d after exposure.The expression level was the highest in +6 Gz group and the lowest in +10 Gz group.Conclusion +Gz exposure can induce obvious changes of HSP70 protein expression level and the most prominent changes are found in rats after exposure to +6 Gz group.