EXPRESSION PROFILES OF NUCLEAR STRUCTURE AND BIOGENESIS-RELATED GENES DURING RAT LIVER REGENERATION / 解剖学报

ABSTRACT

Objective To study the expression changes and profiles of nuclear structure and biogenesis-related genes in rat liver regeneration(LR). Methods Genes involved in nuclear structure and biogenesis were obtained by data collection and literature review.The gene expression changes during LR were checked by Rat Genome 230 2.0 array,and LR-related genes were identified by comparing gene expression difference between partial hepatectomy(PH) and sham operation(SO) groups.Results 406 genes were found to be LR-related.The numbers of initially and totally expressed genes occurring in the forepart(0.5-4 hours after PH) of LR,the prophase(6-12 hours after PH),the metaphase(12-66 hours after PH),and the anaphase(72-168 hours after PH) were 200,29,179,5 and 374,290,1?876,603,respectively.The numbers of their up-regulation and down-regulation were 1?224 and 496 times.The elevated transcription levels occur red to the nuclear organization and biogenesis-involved genes in the prophase and the metaphase of LR and to the nuclear membrane,nuclear matrix and nucleoplasm-involved genes in the metaphase;nuclear chromosome,nucleolus and nuclear functional protein complex-involved genes were up-regulated in the metaphase and the anaphase.Conclusion The expression of nuclear structure and biogenesis-related genes was active during LR,and it was tightly related with LR.