RELEVANCE OF LIPID METABOLISM AND TRANSPORT-INVOLVED GENES TO RAT LIVER REGENERATION / 解剖学报

ABSTRACT

Objective To realize the expression changes and patterns of lipid metabolism and transport-related genes in rat liver regeneration(LR) at transcriptional level. Methods Genes involved in lipid metabolism and transport were obtained by retrieving the databases data and the related papers.The gene expression changes during LR was checked by Rat Genome 230 2.0 array,and LR-associated genes were identified by comparing gene expression difference between partial hepatectomy(PH) and sham operation(SO) groups. Results One hundred ninety three genes were found to be LR-associated.The number of initially and totally expressed genes occurring in forepart(0.5-4 hours after PH) of LR,prophase(6-12 hours after PH),metaphase(12-66 hours after PH),and anaphase(72-168 hours after PH) was 113,20,66,1 and 250,205,796,293,respectively.The number of their up-regulation and down-regulation was 852 and 630 times,and their expression profiles were classified into 27 types.The subdued transcription levels occur to the bile acid metabolism-involved genes in forepart and prophase of LR;the elevated transcription levels occur to the glucocorticoid catabolism-involved genes in forepart and anaphase;phospholipids synthesis-involved genes were up-regulated in prophase and metaphase,while phospholipids catabolism-involved genes were down-expressed;fatty acid,leukotriene and glycosphingolipid synthesis-involved genes,triglyceride and phosphatidylinositol metabolism-involved genes were up-regulated in metaphase,while glycosphingolipid catabolism-involved genes were down-expressed;the elevated transcription levels occur to the prostaglandin synthesis and fatty acid catabolism-involved genes in metaphase and anaphase;during almost the whole period of LR,genes participating in sex hormone,glucocorticoid and progesterone synthesis,sphingomyelin metabolism and lipid transport were up-regulated,while cholesterol metabolism-involved genes were down-expressed.Conclusion Lipid metabolism and transport during LR were diverse,and they were tightly related with LR.