Effect of antibody-blocked HSP70 on the expression of BDNF in injured astrocytes / 重庆医科大学学报

ABSTRACT

Objective:To study its effect on the expression of BDNF from injured astrocytes after HSP70 were blocked by HSP70-antibody. Methods:We performed scratch injuries in the cultured rat astrocytes,and added HSP70-antibody into the nu- trient medium at once after injury(intervention group).Then,at 30min,24h,4d,7d after injury,the expression of brain-derived neurotropic facto(rBDNF)was studied by immunocytochemical and reverse transcriptase-polymerase chain reaction(RT-PCR).The immunocytochemical pictures were analyzed by image analysis system to abtain the average optical density of BDNF. Results: The expression of BDNF increased gradually with injury time.Compared with the control group,the average optical density of BDNF in the intervention group were significantly reduced(P