Determination of Piperine in Root of Piper nigrum L.by HPLC / 中药新药与临床药理
Article
em Zh
| WPRIM
| ID: wpr-579614
Biblioteca responsável:
WPRO
ABSTRACT
Objective To develop a RP-HPLC method for the determination of piperine in the root of Piper nigrum L.Methods RP-HPLC was carried out on Luna C18 column(250 mm? 4.60 mm,5 ? m) with the column temperature of 35 ℃.The mobile phase consisted of a mixture of methanol-water(7723) at a flow rate of 1.0 mL? min-1.The determination wavelength was at 343 nm.Results The calibration curve was linear within the concentration range of 0.164 ? g~ 0.984 ? g,r=0.9996,and the average recovery was 98.09 %,RSD=2.67 %(n=9).The average content of piperine in three batches of pepper roots was in the range of 6.67~6.77mg?g-1.Conclusion Pepper root contains piperine,and this method is suitable for the quality control of the root of Piper nigrum L.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Traditional Chinese Drug Research & Clinical Pharmacology
Ano de publicação:
1993
Tipo de documento:
Article