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Simultaneous determination of caffeic acid,quercetin and campherenol from Hedyotis diffusa Willd by HPLC / 中成药
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-580413
Biblioteca responsável: WPRO
ABSTRACT

AIM:

To establish an HPLC method for determining the contents of caffeic acid,quercetin and campherenol in Hedyotis diffusa Willd.

METHODS:

The samples were separated on an Alltima C 18 (250 mm? 4.6 mm,5 ?m) column with the mobile phase of MeOH(A)-0.5% glacial acetic acid solution;gradient elution(0~15 min,30%~60% A;15~30 min,60%~60% A).Flow rate was 1.0 mL/min. The detection wavelength was set at 350 nm.Column temperature was at 30 ℃.

RESULTS:

The contents of caffeic acid,quercetin and campherenol were 14.218~23.695 ?g/g,9.919~25.564 ?g/g and 6.229~18.160 ?g/g in Hedyotis diffusa Willd from different sources. The linear range of caffeic acid was 0.005 0~0.200 0 ?g(r=0.999 9),the average recovery was 102.35%,RSD was 2.31%(n=6);The linear range of quercetin was 0.006 2~0.244 0 ?g(r=0.999 9),the average recovery was 101.84%,RSD was 1.79%(n=6);The linear range of campherenol was 0.007 8~ 0.310 6 ?g(r=0.999 9),the average recovery was 99.04%,RSD was 2.90%(n=6).

CONCLUSION:

The method for quantifying of caffeic acid,quercetin and campherenol in Hedyotis diffusa Willd is accurate and reliable.

Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Traditional Patent Medicine Ano de publicação: 1992 Tipo de documento: Artigo
Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Traditional Patent Medicine Ano de publicação: 1992 Tipo de documento: Artigo
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