Simultaneous determination of caffeic acid,quercetin and campherenol from Hedyotis diffusa Willd by HPLC / 中成药
Chinese Traditional Patent Medicine
; (12)1992.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-580413
Biblioteca responsável:
WPRO
ABSTRACT
AIM:
To establish an HPLC method for determining the contents of caffeic acid,quercetin and campherenol in Hedyotis diffusa Willd.METHODS:
The samples were separated on an Alltima C 18 (250 mm? 4.6 mm,5 ?m) column with the mobile phase of MeOH(A)-0.5% glacial acetic acid solution;gradient elution(0~15 min,30%~60% A;15~30 min,60%~60% A).Flow rate was 1.0 mL/min. The detection wavelength was set at 350 nm.Column temperature was at 30 ℃.RESULTS:
The contents of caffeic acid,quercetin and campherenol were 14.218~23.695 ?g/g,9.919~25.564 ?g/g and 6.229~18.160 ?g/g in Hedyotis diffusa Willd from different sources. The linear range of caffeic acid was 0.005 0~0.200 0 ?g(r=0.999 9),the average recovery was 102.35%,RSD was 2.31%(n=6);The linear range of quercetin was 0.006 2~0.244 0 ?g(r=0.999 9),the average recovery was 101.84%,RSD was 1.79%(n=6);The linear range of campherenol was 0.007 8~ 0.310 6 ?g(r=0.999 9),the average recovery was 99.04%,RSD was 2.90%(n=6).CONCLUSION:
The method for quantifying of caffeic acid,quercetin and campherenol in Hedyotis diffusa Willd is accurate and reliable.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Traditional Patent Medicine
Ano de publicação:
1992
Tipo de documento:
Artigo