Effect of exogenous PGC -1α on VEGF expression in human retinal vascular endothelial cells / 国际眼科杂志(Guoji Yanke Zazhi)
International Eye Science
; (12): 198-200, 2015.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-637179
Biblioteca responsável:
WPRO
ABSTRACT
AlM To investigate the effect of exogenous peroxisome-proliferator-activated receptor-γcoactivator-1α ( PGC-1α) on vascular endothelial growth factor ( VEGF) expression in human retinal vascular endothelial cells ( HRVEC) .METHODS:
Recombinant PGC-1α protein was added to HRVEC, and no recombinant PGC-1α protein was added to HRVEC as control group. After 24h of incubation, two groups of cells were then placed into a normoxic ( 20%O2 ) or hypoxic ( 1% O2 ) environment for another 16h. The expression of VEGF mRNA and protein were detected by real - time PCR, ELlSA and immunofluorescence cytochemistry.RESULTS:
VEGF mRNA and protein levels in the cells were significantly increased by recombinant PGC - 1αprotein both under normoxia and hypoxia conditions as compared with control groups (P<0. 01).CONCLUSlON PGC-1α can upregulate the expression of VEGF in HRVEC under normoxia and hypoxia conditions.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
International Eye Science
Ano de publicação:
2015
Tipo de documento:
Artigo