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Effect of Weissella cibaria on Fusobacterium nucleatum-induced Interleukin-6 and Interleukin-8 Production in KB Cells
Artigo em Inglês | WPRIM | ID: wpr-67278
Biblioteca responsável: WPRO
ABSTRACT
Oral microorganisms, including pathogens together with commensals, interact with oral epithelial cells, which can lead to the activation and expression of a variety of inflammatory mediators in epithelial cells. Fusobacterium nucleatum is a filamentous human pathogen that is strongly associated with periodontal diseases. Our previous data suggest that Weissella cibaria, an oral commensal, inhibits the proliferation of periodontopathic bacteria including F. nucleatum. The aim of this study was to examine the effects of W. cibaria on the inflammatory mediators, interleukin (IL)-6 and IL-8, in KB cells stimulated by F. nucleatum. In a reverse transcription-polymerase chain reaction and an enzyme-linked immunosorbent assay, live F. nucleatum alone induced high levels of gene expression and protein release of IL-6 and IL-8, whereas W. cibaria alone did not induce IL-6 and IL-8 responses in KB cells. W. cibaria dose-dependently inhibited the increases of the IL-6 and IL-8 gene expression as well as IL-6 protein level in KB cells which was induced by F. nucleatum. Bacterial viability and its coaggregation with F. nucleatum are not essential in the inhibitory effect of W. cibaria. Visible effects of W. cibaria on the attachment and invasion of KB cells by F. nucleatum were observed. In conclusion, W. cibaria may exert immunomodulatory effects on the IL-6 and IL-8 responses to F. nucleatum-activated KB cells.
Assuntos

Texto completo: Disponível Base de dados: WPRIM Assunto principal: Doenças Periodontais / Bactérias / Humanos / Ensaio de Imunoadsorção Enzimática / Células KB / Expressão Gênica / Interleucina-8 / Interleucinas / Interleucina-6 / Fusobacterium nucleatum Idioma: Inglês Revista: Journal of Bacteriology and Virology Ano de publicação: 2011 Tipo de documento: Artigo
Texto completo: Disponível Base de dados: WPRIM Assunto principal: Doenças Periodontais / Bactérias / Humanos / Ensaio de Imunoadsorção Enzimática / Células KB / Expressão Gênica / Interleucina-8 / Interleucinas / Interleucina-6 / Fusobacterium nucleatum Idioma: Inglês Revista: Journal of Bacteriology and Virology Ano de publicação: 2011 Tipo de documento: Artigo
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