Influence of arsenic bisulfide on the expression of HSP70 in K_(562)/ADM cell / 中国癌症杂志

ABSTRACT

Purpose:To study the expression of membrane protein HSP70 of K 562 /ADM cell using arsenic bisulfide(As 2S 2) treatment Methods:Membrane HSP70 and apoptosis of K 562 and multidrug resistant K 562 /ADM cell were studied by flow cytometry assay and RT PCR Results:Membrane HSP70 of K 562 and K 562 /ADM cell gradually increased from 12 to 24 hours with arsenic bisulfide treatment, and had higher expression at the level of HSP70mRNA Expression of membrane HSP70 and apoptosis of K 562 /ADM was higher than that of K 562 Conclusions:Arsenic bisulfide treatment can increase expression of membrane HSP70 of K 562 /ADM and K 562 cells.The change of membrane HSP70 is probably related to K 562 /ADM cell apoptosis