Effect of isoflavone and genistein on expression of CD69 by activated T lymphocytes in vitro / 中国免疫学杂志
Chinese Journal of Immunology
; (12)2000.
Article
em Zh
| WPRIM
| ID: wpr-675155
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To study the effect of isoflavone and genistein on activation of T lymphocytes in order to develope new immuno intervention reagent.Methods:
Fluorescence conjugated monoclonal antibodies and flow cytometer were used to detect the expression rate of CD69 by activated T cells in vitro in response to Phytohemagglutinin(PHA) and Phorbol 12,13 dibutyrate(PDB),with some samples pre incubated with 10,50 or 100 ?mol/L of genistein,after 2 h and 6 h of incubation in whole blood culture system.Results:
After 2 h of culture,the inhibitory effect in PHA group was stronger than PDB group(P
Texto completo:
1
Base de dados:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Immunology
Ano de publicação:
2000
Tipo de documento:
Article