Studies on a high-throughput screening method for identification of HIV fusion inhibitors targeting gp41 / 中国药理学通报
Chinese Pharmacological Bulletin
; (12)1986.
Article
em Zh
| WPRIM
| ID: wpr-677951
Biblioteca responsável:
WPRO
ABSTRACT
AIM To modify and improve a screening assay so that it becomes more convenient, economic and adaptable in China for high throughput screening of HIV fusion inhibitors targeting gp41. METHODS The original screening method reported by Jiang et al (J Virol. Methods 1999;80:85 96) was modified by: ① using a conformation specific monoclonal antibody to replace a polyclonal antibody for coating plates; ②simplifying the procedures; ③using parts of the reagents produced in China. RESULTS The modified screening assay is simpler, more convenient, and more economic than the original assay, but its sensitivity is comparable to and specificity is a little better than the original method. CONCLUSIONS The modified screening assay is more convenient and economic and can be used in China for high throughput screening of HIV fusion inhibitors from complex sample, such as phage display peptide libraries, microorganism fermentation liquids, herbs and other natural products.
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1
Base de dados:
WPRIM
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Idioma:
Zh
Revista:
Chinese Pharmacological Bulletin
Ano de publicação:
1986
Tipo de documento:
Article