STUDY ON THE HYPOGLYCEMIC ACTION OF PUMPKIN EXTRACT IN DIABETIC RAT / 营养学报

ABSTRACT

Objective: To study the hypoglycemic action in diabetic rats by soluble polysaccharides (A), protein components with molecular weights above 60 ku (B),3-60 ku(C) ,and below 3 ku(D) of some pumpkin seeds,arginine(E)and pumpkin seed oil(F). Methods: Ten rats were selected randomly as control ,while the other 150 rats were injected with alloxan 170 mg/kg to induce diabetes.The diabetic rats were divided into 15 groupsthe model,A(1000,500 mg/kg),B(300,150 mg/kg),C(300,150 mg/kg),D(300,150 mg/kg),E(100,50 mg/kg),F(300,150 mg/kg),Gilbenclamide(20 mg/kg).The above components were given intragastrically for 10 d and then blood samples were taken to determine serum glucose and insulin, and also to test the glucose tolerance. Results: It was indicated in diabetic rat that the blood insulin level was obviously increased by the soluble polysaccharides , the protein components with molecular weight over 60 ku and below 3 ku after 4 d germination. The blood glucose tolerance was improved by seed oil, protein components with molecular weights 3-60 ku and arginine. Conclusion: The protein components with molecular weights about 3-60 ku have the best hypoglycemic action, in terms of improving the blood glucose tolerance and decreasing the blood glucose level of diabetic rats.