Effect of epidermal growth factor on proliferation and migration ability of amelanotic melanocytes in human hair follicle outer root sheath / 重庆医学

ABSTRACT

Objective To investigate the effect of epidermal growth factor (EGF) on proliferation and migration ability of amelanotic melanocytes (AMMC) in human hair follicle outer root sheath.Methods AMMC were purified to the third generation by the variant enzyme method.Then AMMC was identified by the immunohistochemical method;the changes of cellular proliferation and migration ability after intervention by different concentrations of EGF were detected by using CCK8 and Tianswell in vitro chamber migration test.Results The third generation AMMC immaro histochemical staining showed NKI/beteb staining positive and HMB-45 staining negative.The OD values of CCK8 test and the Transwell in vitro chamber migration assay results showed that its promoting proliferation and migration effects were increased with the EGF concentration increase,but when further increasing concentration,the effects had no obvious enhancement.Conclusion A certain concentration range of EGF in vitro can promote AM-MC proliferation and migration.