Long time exposure to low concentrations of sodium arsenite induces Warburg effect in human bladder epithelial cells / 中华地方病学杂志

ABSTRACT

Objective To study the influence of long time exposure to low levels of sodium arsenite on Warburg effect in cultured human epithelial cells (SV-HUC-1) at different times.Methods SV-HUC-1 cells were exposed to 0.5 μmol/L sodium arsenite for 10,20,30 weeks and cells cultured without sodium arsenite for 10,20,30 weeks were regarded as control groupsin vitro.Lactate assay kit and glucose assay kit were used to measure the lactate secretion and glucose consumption levels,and cells mRNA and protein expressions of SCL2A1 and hexokinase2(HK2) were detected using Real-time PCR and Western blotting.Results The levels of lactate secretion [(4.67 ± 0.20),(7.47 ± 0.28),(12.46 + 0.47) mmol/L],glucose consumption [(2.86 ± 0.11),(4.25 ± 0.19),(6.38 ± 0.05) mmol/L] and expression of HK2 protein (1.21 ± 0.06,1.36 ± 0.13,1.60 ± 0.12) increased significantly after treated with 0.5 μmol/L sodium for 10,20,30 weeks compared with those of control groups [(3.04 ± 0.11),(3.90 ± 0.32),(4.77 ± 0.24) mmol/L;(2.17 ± 0.15),(2.48 ± 0.24),(2.71 ± 0.13) mmol/L;1.00 ± 0.00;P ＜ 0.05].Compared with control group,the expressions of SCL2A1 mRNA,HK2 mRNA and SCL2A1 protein in SV-HUC-1 cells treated with sodium arsenite for 10 weeks increased but the difference was not statistically significant (P ＞ 0.05).While the expressions of SCL2A1 mRNA,HK2 mRNA and SCL2A1 protein in SV-HUC-1 cells treated with sodium arsenite for 20 and 30 weeks increased significantly compared to those of control groups (P ＜ 0.05).Conclusion Long-term exposure to low concentrations of sodium arsenite can increase glycolysis in SV-HUC-1 and induce Warburg effect.