Polyphenylene-polysaccharide complex from pinus koraiensis extract induces apoptosis in human breast cancer MCF-7 cells / 实用肿瘤学杂志

ABSTRACT

Objective The mechanism of apoptosis induced by polyphenyl propenoid-polysaccharide complex(PPC)in hu-man breast cancer MCF-7 cells was studied. Methods MCF-7 cells were cultured with different concentrations of PPC for differ-ent time. The effects of PPC on proliferation and apoptosis were detected in MCF-7 cells by MTT assay,fluorescent staining,apopto-sis detection kit,DNA gel electrophoresis and Western blot. Results PPC induced apoptosis in MCF-7 cells. When apoptosis oc-curred,the enzyme activities of caspase-3 and-9 were increased,and the expression of pro-caspase-3 protein was decreased. Caspase-3 inhibitor(z-DEVE-fmk)could partially inhibit PPC-induced apoptosis and inhibit activation of caspase-3 precursor enzyme. Conclusion PPC induces apoptosis of MCF-7 cells by activating caspase family.