Direct Conversion of Human Umbilical Cord Blood into Induced Neural Stem Cells with SOX2 and HMGA2
International Journal of Stem Cells
; : 227-234, 2017.
Article
em En
| WPRIM
| ID: wpr-80749
Biblioteca responsável:
WPRO
ABSTRACT
Recent advances have shown the direct reprogramming of mouse and human fibroblasts into induced neural stem cells (iNSCs) without passing through an intermediate pluripotent state. Thus, direct reprogramming strategy possibly provides a safe and homogeneous cellular platform. However, the applications of iNSCs for regenerative medicine are limited by the restricted availability of cell sources. Human umbilical cord blood (hUCB) cells hold great potential in that immunotyped hUCB units can be immediately obtained from public banks. Moreover, hUCB samples do not require invasive procedures during collection or an extensive culture period prior to reprogramming. We recently reported that somatic cells can be directly converted into iNSCs with high efficiency and a short turnaround time. Here, we describe the detailed method for the generation of iNSCs derived from hUCB (hUCB iNSCs) using the lineage-specific transcription factors SOX2 and HMGA2. The protocol for deriving iNSC-like colonies takes 1~2 weeks and establishment of homogenous hUCB iNSCs takes additional 2 weeks. Established hUCB iNSCs are clonally expandable and multipotent producing neurons and glia. Our study provides an accessible method for generating hUCB iNSCs, contributing development of in vitro neuropathological model systems.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Fatores de Transcrição
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Cordão Umbilical
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Técnicas In Vitro
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Neuroglia
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Medicina Regenerativa
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Células-Tronco Neurais
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Sangue Fetal
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Fibroblastos
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Métodos
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Neurônios
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
Idioma:
En
Revista:
International Journal of Stem Cells
Ano de publicação:
2017
Tipo de documento:
Article