Expression of FNDC5/Irisin in pancreatic cancer and its relationship with clinicopathological features / 中华肝胆外科杂志

ABSTRACT

0bjective To investigate the expression and distribution of FNDC5/Irisin in pancreatic cancer tissues and adjacent tissues.and to analyze its correlation with clinicopathological features.Methods Collection of archived wax blocks from 64 patients diagnosed with pancreatic cancer after surgical treatment from January 2015 to December 2018 in the Department of Pathology,Affiliated Qingdao Municipal Hospital of Qingdao University,and 30 tissues collected intraoperatively from January 2016 to December 2018 Sam-ples,all collected samples included tumor tissue and corresponding adjacent tissues(＞2 am from the tumor edge).Real-time quantitative PCR(qRT-PCR)and immunohistochemistry were used to detect the expres-sion of FNDC5/Irisin mRNA and its positivity in pancreatic cancer tissues and adjacent tissues.and to ana-1yze its relationship with clinicopathological features of pancreatic cancer.Results qRT-PCR showed that the expression of FNDC5/Irisin mRNA in pancreatic cancer tissues was higher than that in the corresponding adjacent pancreatic tissues,the difference was statistically significant(P＜0.05).The immunohistochemis-try results showed that the positivity of FNDC5/Irisin in pancreatic cancer tissues was 59.4％.and the posi-tivity of FNDC5/Irisin in adjacent tissues was 28.1％.and the positivity of FNDC5/Irisin in cancer tissues and adjacent pancreatic tissues was significantly different(P＜0.05)the expression level of FNDC5/Irisin was not related with the gender,age,tumor size,degree of differentiation,and tumor stage.(P＞0.05),but FNDC5/Irisin expression was associated with liver and lymph node metastasis(P＜0.05).Conclusion The positivity of FNDC5/Irisin in pancreatic cancer tissues is significantly higher than that in adjacent pan-creatic tissues.and it is correlated with liver and lymph node metastasis in pancreatic cancer.