Effect of siRNA targeting TGF-β inducible early gene on expression of advanced glycation end products-mediated Smad2 in renal tubular epithelial cells / 第二军医大学学报
Academic Journal of Second Military Medical University
; (12): 354-358, 2010.
Article
em Zh
| WPRIM
| ID: wpr-840324
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To investigate the effect of siRNA targeting TGF-β inducible early gene (TIEG) on expression of Smad2 in advanced glycation end products (AGEs)-mediated renal tubular epithelial cells.Methods:
The pshRNA-copGFP-lentivector containing target gene was used as a vector to construct siRNA-TIEG, which was then transfected into normal rat proximal tubular epithelial cells(NRK52E), which were then cultured in RPMI 1640 medium supplemented with AGE-BSA for 24 h and 48 h. The expression of Smad2 mRNA and protein was examined by PCR, Western blotting analysis, respectively. Blank vector group served as control.Results:
SiRNA-TIEG significantly reduced the expression of TIEG mRNA, Smad2 mRNA and Smad2 protein in normal rat proximal tubular epithelial cells in presence of AGE-BSA compared with those in the control group (P<0.05).Conclusion:
Silence of TIEG can effectively inhibit the expression of Smad2 mediated by AGEs in NRK52E cells.
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Base de dados:
WPRIM
Idioma:
Zh
Revista:
Academic Journal of Second Military Medical University
Ano de publicação:
2010
Tipo de documento:
Article