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Role of JNK pathway and HSP70 in therono-chemotherapy of lung cancer / 第二军医大学学报
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-840938
Biblioteca responsável: WPRO
ABSTRACT

Objective:

To study the effect of thermo-chemotherapy on lung cancer and its possible mechanism.

Methods:

H446 cells were subjected to different thermo-chemotherapy strategies 43°C + Paclitaxel (120 μg/L, thermo-chemotherapy group), 43°C+Paclitaxel (120 μg/L)+SP600125 (20 μmol/L, JNK inhibitor) (thermo-chemotherapy+SP600125 group), thermotherapy (43°C) group, and Paclitaxel (120 μg/L) group; untreated cells served as control. MTT assay was used to measure cell proliferation and Western blotting was used to examine the expression of JNK, p-JNK and HSP70 protein.

Results:

The proliferation rate of cells in the thermo-chemotherapy group was significantly lower than those in the other 3 groups (all P<0.05). The expression of p-JNK was significantly increased in the thermo-chemotherapy group (P<0.05); SF600125 inhibited the expression of p-JNK and the proliferation of cells in the thermo-chemotherapy+ SP600125 group was elevated (P< 0.05). The expression of HSP70 in the thermo-chemotherapy group was lower than that of the thermotherapy group (P<0.05).

Conclusion:

Thermotherapy can obviously promote the inhibitory effect of Paclitaxel chemotherapy against the growth of lung cancer cell line H446, probably through activating JNK pathway or inhibiting expression of HSP70 protein.

Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Academic Journal of Second Military Medical University Ano de publicação: 2010 Tipo de documento: Artigo
Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Academic Journal of Second Military Medical University Ano de publicação: 2010 Tipo de documento: Artigo
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