Changes of hepatic caspase-3 and caspase-8 activities during liver regeneration in rats / 第二军医大学学报

ABSTRACT

Objective: To study the changes of hepatic caspase-3 and caspase-8 activities during liver regeneration in rats. Methods: Liver regenerati on model was produced by 70% partial hepatectomy (PH) in male SD rats. Then the rats were sacrificed and the liver homogenate were prepared. Hepatic caspase-3 and caspase-8 activities were determined at 8 different time points during the liver regeneration at the absorbance of 405 nm. Results: Hepatic caspase-3 activity increased significantly 3 h, 6,72 h, 120 h and 168 h after PH compared with that of control group(P<0.001); Hepatic caspase-8 activity increased 168 h after PH(P<0.05) and had no obvious change at any other time points. Conclusion: Our result suggests that the increase of caspase-3 activity during the liver regeneration may be mainly induced by the mitochondrial pathway.