TLC identification of quercetin and chlorogenic acid in Ramulus mori and their antioxidant activity screening / 国际药学研究杂志
Journal of International Pharmaceutical Research
; (6): 548-551, 2016.
Article
em Zh
| WPRIM
| ID: wpr-845560
Biblioteca responsável:
WPRO
ABSTRACT
Objective To establish a thin-layer chrometograrrgy(TLC) method for the identification of quercetin and chlorogenic acid in Ramulus mori, and to screen their antioxidant activity. Methods The quercetin and chlorogenic acid were extracted by ultrasonic method with methanol as a solvent. The effect of different developed system, reagent, temperature, view methods and different silica gel plate on the TLC of quercetin and chlorogenic acid in Ramulus mori were tested to select the best TLC conditions. The antioxidant activity of quercetin and chlorogenic acid was screened with DPPH as a reagent. Results The ethyl acetatewaterformic acidtoluene(17220.8) was used as a developing system and 1% AlCl3 as a chromogenic reagent. Quercetin and chlorogenic acid in Ramulus mori were identified under 366 nm, with blue and blue-green spots on silica gel G plate, and yellowish spots under purple background by the test of TLC-bioautography. Both were proven to have antioxidant activity. Conclusion The method is simple, accurate and reliable, and can be used for quality control of Ramulus mori.
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1
Base de dados:
WPRIM
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Idioma:
Zh
Revista:
Journal of International Pharmaceutical Research
Ano de publicação:
2016
Tipo de documento:
Article