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Bioactivity of the peptide P17-bone morphogenetic protein-2/nano-hydroxyapatite-mineralized collagen composite / 中国组织工程研究
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-847702
Biblioteca responsável: WPRO
ABSTRACT

BACKGROUND:

A mineralized collagen composite, i.e. nano-hydroxyapatite/collagen (nHAC) has biomimetic three-dimensional structure and good bioactive properties. As a bone tissue engineering material, it is widely used in bone defect repair. A newly designed P17-bone morphogenetic protein-2 (P17-BMP2) has good biocompatibility and osteogenic capacity. Therefore, the composite scaffold material was prepared by combining the new P17-BMP-2 and nHAC, which might be used for the enhancement of osteogenic capacity in the treatment of bone defects.

OBJECTIVE:

To investigate the bioactivity of the P17-BMP-2/nHAC composite.

METHODS:

Rabbit bone marrow mesenchymal stem cells were seed on the P17-BMP-2/nHAC composite and nHAC. After 3 and 7 days of culture, the relative expression level of alkaline phosphatase was detected by RT-PCR. The subcutaneous implantation of P17-BMP-2/nHAC (experimental group) and nHAC (control group) into Sprague-Dawley rats was performed. Masson staining was performed for histological analysis at 12 and 35 days of implantation. P17-BMP-2/nHAC (experimental group) and nHAC (control group) were implanted into the white rabbit mandibular box-shaped bone defect, respectively. At 5 and 15 weeks, gross observation and X-ray were performed. The study was approved by the Medical Ethics Committee of China Medical University School & Hospital of Stomatology. RESULTS AND

CONCLUSION:

(1) The relative expression level of alkaline phosphatase in the P17-BMP-2/nHAC group was significantly higher than that in the nHAC group (P < 0.05). (2) The result of subcutaneous implantation showed that the acute inflammatory response initiated by the P17-BMP-2/nHAC or nHAC was not found. More activated fibroblasts growing into the implants could be found on the sections of P17-BMP-2/nHAC compared to that of nHAC at 35 days after implantation. (3) In the bone defect repair test, gross observation showed that both materials held good defect repair ability, the defect area began to reduce at 5 weeks after implantation, and the defect surface became flat at 15 weeks after implantation. X-ray examination showed that compared with the control group, the defect area was more significantly reduced in the experimental group. (4) These results indicate that P17-BMP-2/nHAC composite scaffold has higher bioactivity and a stronger ability to repair bone defect.

Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Aspecto: Aspectos éticos Idioma: Chinês Revista: Chinese Journal of Tissue Engineering Research Ano de publicação: 2020 Tipo de documento: Artigo
Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Aspecto: Aspectos éticos Idioma: Chinês Revista: Chinese Journal of Tissue Engineering Research Ano de publicação: 2020 Tipo de documento: Artigo
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