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Characterization of self-assembled bone-like nano-hydroxyapatite/collagen composites / 中国组织工程研究
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-847913
Biblioteca responsável: WPRO
ABSTRACT

BACKGROUND:

The materials that are similar to natural bone and can be used for repair of bone defects or as tissue engineering material have become the focus of research.

OBJECTIVE:

To investigate the feasibility of self-assembly of nano-hydroxyapatite collagen composite.

METHODS:

Collagen was immersed in 0.25% glutaraldehyde solution for 0.5 (group A), 24 (group B) and 72 hours (group C) for cross-linking reaction. In the group D, collagen was immersed in carbodiimide for 4 hours. After cross-linking reaction, collagen in each group was immersed in nano-hydroxyapatite solution for 7 days to prepare bone-like composite materials. The mineral phase analysis, composition, and microstructure of composite materials and natural bone were analyzed. RESULTS AND

CONCLUSION:

X-ray diffraction

analysis:

The amorphous diffraction peaks of the composites were slightly higher than those of the natural bone. The amorphous change of the composite was not obvious. With the prolongation of glutaraldehyde cross-linking time, the peak value of the crystal of the composite tended to increase. The amorphous diffraction peaks of the composites crosslinked with carbodiimide were slightly lower than those crosslinked with glutaraldehyde. Fourier transform infrared spectroscopy

analysis:

The chemical composition of the composites was similar to that of the natural bone, which was composed of collagen and hydroxyapatite, in which PO43-was partly replaced by CO32-ion in hydroxyapatite. Different cross-linking methods had no obvious effects on inorganic phase change of the materials. Scanning electron microscopy The effects of different collagen cross-linking methods on the morphology of the formed crystals were not significantly different. Collagen fibers intertwined. A large number of fine needle-like crystals deposited on them, clustered, and were uniformly distributed. The crystal was nano-scaled in size. These results suggest that bone-like composite can be self-assessmbled with nano-hydroxyapatite precursor and collagen.

Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Journal of Tissue Engineering Research Ano de publicação: 2020 Tipo de documento: Artigo
Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Journal of Tissue Engineering Research Ano de publicação: 2020 Tipo de documento: Artigo
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