Simultaneous determination of six terpenoids in Crotonis Crassifolii Radix by HPLC / 中草药

ABSTRACT

Objective: To establish an HPLC method for simultaneous determination of chettaphanin I, teucvidin, crassifolin B, cyperenoic acid, crassifolin A, and cyperenol in Crotonis Crassifolii Radix. Methods: The separation was performed on a Kromasil 100-5 C18 column (250 mm×4.6 mm, 5 μm) with acetonitrile (A)-0.02% trifluoroacetic acid water (B) as the mobile phase in a gradient elution (0-35 min, 35% A; 35-55 min, 35%-60% A; 55-80 min, 60% A) at a flow rate of 1.0 mL/min. The detective wavelength was set at 210 nm, and the column temperature was set at 25℃. Results: The six terpenoids all have good separating degree and good linearity (r>0.999 7). The sample recoveries of compounds 1-6 were 100.2% (RSD=0.48%), 99.13% (RSD=0.48%), 98.48% (RSD=0.96%), 99.22% (RSD=1.10%), 101.1% (RSD=1.35%), and 102.5% (RSD=0.95%), respectively. Conclusion: The established method is rapid and accurate, and has high repeatability, which could provide the scientific evidence for the quality control of Crotonis Crassifolii Radix.