Establishment of fingerprint for Xinjiang chrysanthemum and analysis on its components by UPLC / 中草药

ABSTRACT

Objective: In order to identify the authenticity and control the quality of Xinjiang chrysanthemum, the fingerprinting of it was established using UPLC and discussed in this paper. Methods: Thirteen batches of Xinjiang chrysanthemum from different origins were analyzed by UPLC with catechin, luteoloside, quercetin, and 3, 5-di-O-caffeoyl quinic acid as reference substances. The chromatographic condition was performed on Waters ACQUITY UPLC HSS T3 C18 (150 mm × 2.1 mm, 1.8 μm) and eluted with mobile phase containing acetonitrile-pH 3.0 phosphoric acid solution. The flow rate was 0.2 mL/min with detection wavelength 280 nm in the temperature of 30℃. Results: There were 17 peaks in the UPLC fingerprint of Xinjiang chrysanthemum and three components were identified by three peaks determined using reference substance. Conclusion: The similarity of 13 batches is little difference, but is more than 0.90. The results showed that the UPLC fingerprint chromatogram could be used as a valuable method for the quality evaluation of Xinjiang chrysanthemum.