Effects of thin recipe of Buyang Huanwu decoction on CDK5 and apoptotic factors in an oxidative stress cell model / 中国药理学通报

ABSTRACT

Aim To explore the neuroprotective mechanism of thin recipe of Buyang Huanwu decoction on CDK5 and apoptotic factors in an oxidative stress cell model. Methods A PC 12 cell model of oxidative stress damage was established using hydrogen peroxide (H202) at the concentration of 0. 3 mmol • L"1 co-cultured for 2 h, PC 12 cells were divided into four groups, namely normal control, model control, Buyang Huanwu decoction and its thin recipe groups. We evaluated the content of extracellular L-LDH and intracellular GSH by ELISA, and the extracellular ROS levels by FCM. In addition, the expressions of CDK5 and Tau were further analyzed to facilitate the understanding of neuroprotective mechanism by IF, and the mR-NA levels of apoptotic factors such as caspase-3 and the ratio of Bax/Bcl-2 mRNA were determined by qPCR. Results The thin recipe of Buyang Huanwu decoction increased GSH activity, decreased L-LDH and ROS levels compared with model cells at the concentration of 1.0 g • L"1 (P < 0. 05). Furthermore, we observed lower expression of CDK5 and Tau, as well as less mRNA of caspase-3 and the ratio of Bax/Bcl-2 in the thin recipe of Buyang Huanwu decoction groups than that of model groups (P < 0. 05). Conclusions The thin recipe of Buyang Huanwu decoction could play the neuroprotective effects by CDK5 and Tau signal sites to inhibit neuronal apoptosis after oxi-dative stress damage of PC12, which illuminate the partial mechanism and the reasonability of this new recipe simplified from Buyang Huanwu decoction in molecular level.