Effects of sodium quercetin-7-sulfate on recombinant human protein kinase CK2 holoenzyme / 中国药理学通报
Chinese Pharmacological Bulletin
; (12): 315-318, 2002.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-857495
Biblioteca responsável:
WPRO
ABSTRACT
AIM:
To study the effects and kinetics of sodium quercetin-7-sulphate (SQMS) on recombinant human protein kinase CK2 holoenzyme.METHODS:
The recombinant human CK2 holoenzyme activity was assayed by detecting incorporation of 32p of [γ-32P]ATP into the substrate at various conditions.RESULTS:
SQMS was shown to strongly inhibit the holoenzyme activity of recombinant human protein kinase CK2 with an IC50 of 1.37 μmol·L-1, Kinetic studies of SQMS on recombinant human CK2 showed the inhibition was competitive with ATP and noncompetitive with casein.CONCLUSION:
SQMS is an inhibitor of protein kinase CK2, and the inhibitory action of SQMS on CK2 is competitive with ATP and noncompetitive with casein.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Pharmacological Bulletin
Ano de publicação:
2002
Tipo de documento:
Artigo