Anti-obesity Effects of Alcohol Extract from Abelmoschus esculentus Linn. and Its Correlation with Gene SREBP-1 and FAS / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the anti-obesity effect of crude extract of okra on nutrition-obese mice. METHODS: The fifty SPF ICR male mice were randomly divided into five groups control(0.9% normal saline), model(0.9% normal saline), okra alcohol extraction high, medium and low dose group(400, 200, 100 mg·kg-1). In addition to the mice in control group, the mice in other four groups were given high fat diet to make the model of obese mice, after oral administration for 4 weeks, eyeball blood of the mice was taken to measure the contents of serum triglyceride(TG), total cholesterol(TC), leptin(LEP), adiponectin(ADP), high density lipoprotein(HDL-C), and low density lipoprotein(LDL-C). Then mice were sacrificed to take their liver tissue and fat to detect the expression of SREBP-1 and FAS mRNA by RT-PCR. RESULTS: Compared with the model, the okra alcohol extract could effectively decrease the contents of TC, TG, LDL-C and LEP, increase the contents of HDL-C and ADP(P<0.01, P<0.05), improve the dyslipidemia, and regulate the secretion of protein in adipose tissue, to achieve the therapeutic effect on obese mice. The expressions of SREBP-1 and FAS mRNA were also significantly inhibited by crude extract of okra. CONCLUSION: The results show that treatment of crude extract of okra on obese mice is associated with the expressions of SREBP-1 and FAS.