Determination of Phenanthrenes in the Anxiolytic Fraction of Juncus effusus L. by QNMR / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish a method for quantification of phenanthrenes in the anxiolytic fraction of Juncus effusus L by QNMR. METHODS: The analysis was conducted using terephthalic acid as an internal standard, the 5 mm SEI probe temperature was 298.2 K with NS=16 and d1=1 s. C4-H of dehydroeffusol, C4-H of effusol and C4-H juncusol peaks were chosen as the characteristic peaks. RESULTS: The contents of effusol, dehydroeffusol, juncusol and dehydrojuncusol were 338.75, 41.14 and 109.13 mg·g-1, respectively, by QNMR, and 340.87, 42.99, 107.73 and 9.561 mg·g-1, respectively, by HPLC. CONCLUSION: The determined contents of phenanthrenes by QNMR are in accordance with the results by HPLC, indicating that QNMR can be applied to determine phenanthrenes in the anxiolytic fraction of Juncus effusus L. QNMR is rapid, accurate, and does not need reference substances and complex sample preparation.