Anti-fatigue effect of dihydromyricetin on mice / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the anti-fatigue effect of dihydromyricetin on mice and its anti-fatigue mechanism. METHODS: Forty male mice were randomly divided into blank control group and dihydromyricetin dose groups (40, 20, 10 mg·kg-1), with continual intragastric administration for 28 d. Through the weight loading swimming experiment of mice, the swimming time of mice were recorded. On the twenty-nine day, mice swam 90 min without loading, and then the activities of BUN, BLA in the serum of mice and biochemical indicators of SOD, GSH-Px, MDA and hepatic glycogen etc were respectively determined. The expression of mRNA of PGC-1α and PPARα were detected by real-time PCR. RESULTS: Comparing with the blank control group, dihydromyricetin dose group can prolong the loaded-swimming time of mice, increase the reserve of liver glycogen in the body, reduce levels of BLA, BUN and MDA, and significantly increased the activities of SOD and GSH-Px. RT-PCR RESULTS showed that the expression levels of PGC-1α and PPARα mRNA in skeletal muscle of mice in the dihydromyricetin dose group were significantly increased, and all had statistical significance. CONCLUSION: Dihydromyricetin can accelerate clearance of free radical, enhance mice antioxidant capacity and improve exercise ability in fatigued mice thus play the anti-fatigue role, exercise-induced fatigue is correlated with PGC-1α.