Determination of quercetin, podophyllotoxin and kaempferol in Diphylleia sinensis Li. by HPLC / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish a method to determine quercetin, podophyllotoxin and kaempferol in Diphylleia sinensis Li. METHODS: Diamonsil C18 column was used. Methanol-0.4% glacial acetic acid solution was used as the mobile phaseat a flow rate of 1 mL · min-1. The column temperature was 30°C. The detection wavelength was 290 nm. RESULTS: The liner ranges of quercetin, podophyllotoxin and kaempferol were 0.023-0.287 μg (r=0.999 9), 0.208-2.6 μg (r=0.999 9), and 0.036 1-0.451 μg (r=0.999 9), respectively. The average recoveries were 99.64% (RSD=0.83%), 100.04% (RSD=0.61%), and 99.88% (RSD=1.19%), respectively. CONCLUSION: The method is sensitive, accurate and reproducible. It can be used for the determination of quercetin, podophyllotoxin and kaempferol in Diphylleia sinensis Li.