Dependence of RIG-I Nucleic Acid-Binding and ATP Hydrolysis on Activation of Type I Interferon Response
Immune Network
; : 249-255, 2016.
Article
em En
| WPRIM
| ID: wpr-97829
Biblioteca responsável:
WPRO
ABSTRACT
Exogenous nucleic acids induce an innate immune response in mammalian host cells through activation of the retinoic acid-inducible gene I (RIG-I). We evaluated RIG-I protein for RNA binding and ATPase stimulation with RNA ligands to investigate the correlation with the extent of immune response through RIG-I activation in cells. RIG-I protein favored blunt-ended, double-stranded RNA (dsRNA) ligands over sticky-ended dsRNA. Moreover, the presence of the 5'-triphosphate (5'-ppp) moiety in dsRNA further enhanced binding affinity to RIG-I. Two structural motifs in RNA, blunt ends in dsRNA and 5'-ppp, stimulated the ATP hydrolysis activity of RIG-I. These structural motifs also strongly induced IFN expression as an innate immune response in cells. Therefore, we suggest that IFN induction through RIG-I activation is mainly determined by structural motifs in dsRNA that increase its affinity for RIG-I protein and stimulate ATPase activity in RIG-I.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
RNA
/
RNA de Cadeia Dupla
/
Ácidos Nucleicos
/
Interferon Tipo I
/
Trifosfato de Adenosina
/
Adenosina Trifosfatases
/
Hidrólise
/
Imunidade Inata
/
Ligantes
Idioma:
En
Revista:
Immune Network
Ano de publicação:
2016
Tipo de documento:
Article