Elevated prevalence of high-risk HPV in healthy oral mucosa of users and nonusers of drugs in Northeastern Brazil

Abstract: Variable rates of HPV infection have been reported in healthy oral mucosa worldwide. The main objective of this study was to detect and genotype HPV infection in users and nonusers of drugs with clinically healthy mucosa from the Northeast Brazil. Samples from 105 patients were amplified using the primers MY09/MY11 and GP5+/GP6+, and genotyping was performed by multiplex-PCR for HPV-6/11, 16 and 18. A total of 81.9% samples were positive. Among drug users, 84.5% presented the virus and 20.4% showed multiple infections. Among non-drug users, 78.7% were positive and 13.5% had multiple infections. Limited information is available on oral HPV in Brazilian population, especially for drug users, and our results showed higher HPV infection rates in both users and nonusers of drugs. More studies and researches focused on drug users including factors like sexual behavior, nutrition and cultural habits are necessary to enhance the comprehension of this relationship, and develop preventive strategies.

High prevalence of human papillomavirus (HPV) in oral mucosal lesions of patients at the Ambulatory of Oral Diagnosis of the Federal University of Sergipe, Northeastern Brazil

Abstract The role of human papillomavirus (HPV) in oral carcinogenesis is still controversial as detection rates of the virus in oral cavity reported in the literature varies greatly. Objective The aim of this study was to evaluate the frequency of HPV infection and its genotypes in patients with oral lesions at the Ambulatory of Oral Diagnosis of the Federal University of Sergipe, Brazil. Material and Methods We conducted a molecular study with 21 patients (15 females) aged from two to 83 years with clinically detectable oral lesions. Samples were collected through exfoliation of lesions and HPV-DNA was identified using MY09/11 and GP5+/6+ primers. Genotyping was performed by multiplex PCR. Results Benign, premalignant and malignant lesions were diagnosed by histopathology. HPV was detected in 17 samples. Of these, HPV-6 was detected in 10 samples, HPV-18 in four and HPV-16 in one sample. When samples were categorized by lesion types, HPV was detected in two papilloma cases (2/3), five carcinomas (5/6), one hyperplasia (1/1) and nine dysplasia cases (9/11). Conclusion Unlike other studies in the literature, we reported high occurrence of HPV in oral lesions. Further studies are required to enhance the comprehension of natural history of oral lesions.

Mortality from oral cancer in Aracaju/SE, Brazil: 2000-2009 / Mortalidade por câncer de boca em Aracaju/SE, Brasil: 2000-2009

Introduction: The incidence of oral cancer varies widely in the world, with two thirds of cases diagnosed in developing countries. Objective: To investigate the epidemiology of deaths from oral cancer and the spatial distribution in the city of Aracaju in the state of Sergipe between 2000 and 2009. Material and Method: Data were collected using the Mortality Information System (MIS) of Aracaju/SE at the DATASUS website and were tabulated using the software TABWIN 3.4. The geographic distribution of deaths by neighborhoods of that city was visualized. Result: Of the 78 deaths, 61 (78.2%) were male. The mean age was 64.23 years. Individuals of mixed ethnicity had a higher death frequency (34.6%). Regarding education, the majority of death certificates recorded the option ignored, followed by individuals with 1 to 3 years of study. The anatomical sites most affected were unspecified parts of the mouth (43.6%). The spatial distribution analysis indicated that neighborhoods such as Santos Dumont, Luzia, Atalaia, Santa Maria, Cidade Nova and Industrial had the highest death frequencies. Conclusion: Males between the fifth and sixth decades of life of mixed ethnicity, low education and in neighborhoods with low and very low standards of living constitute the epidemiological profile for deaths from oral cancer.

Introdução: A incidência do câncer de boca varia amplamente no mundo, sendo que dois terços dos casos têm sido diagnosticados em países em desenvolvimento. Objetivo: Investigar a epidemiologia dos óbitos por câncer de boca e a distribuição espacial no município de Aracaju, estado de Sergipe, entre 2000 e 2009. Material e Método: Os dados foram coletados do Sistema de Informação de Mortalidade (SIM) de Aracaju/SE e no site do DATASUS e foram tabulados através do Tabwin 3.4. Foi realizada a distribuição geográfica dos óbitos por bairros desta cidade. Resultado: Dos 78 óbitos, 61 (78,2%) eram do sexo masculino. A média de idade foi 64,23 anos. A maior frequência de óbitos foi em indivíduos de cor parda (34,6%). Quanto à escolaridade, a maioria das declarações de óbito registrou a opção ignorada, seguida de indivíduos com 1 a 3 anos de estudo. Os sítios anatômicos mais acometidos foram partes não especificadas da boca (43,6%). A análise da distribuição espacial mostrou que os bairros Santos Dumont, Luzia, Atalaia, Santa Maria, Cidade Nova e Industrial apresentaram as maiores frequências de óbito. Conclusão: O perfil epidemiológico delineado foi de indivíduos do sexo masculino, entre a quinta e sexta décadas de vida, de cor parda, baixa escolaridade e nos bairros com baixa ou muito baixa condição de vida.