Knowledge, Attitudes, and Practices Regarding Teledentistry Among Dentists in Jakarta During the Coronavirus Disease (Covid-19) Pandemic: A Cross-Sectional Study

Abstract Objective: To assess factors associated with knowledge, attitudes, and practices (RAP) related to teledentistry among dentists in Jakarta during the COVID-19 pandemic. Material and Methods: This cross-sectional study used a self-administered online questionnaire with a purposive sampling technique. Survey items comprised knowledge of, attitude toward, the practice of, and barriers to teledentistry. The Chi-square, Mann-Whitney, and Spearman correlation tests were used to determine factors associated with RAP of teledentistry. Results: A total of 183 dentists in Jakarta completed the questionnaire. The findings showed that 95.6% of the surveyed dentists had good knowledge, 83.1% expressed positive attitudes, and 60.7% practiced teledentistry during the COVID-19 pandemic. Most participants (86.3%) expressed a willingness to practice teledentistry in the future. The three most significant barriers that obstruct dentists from practicing teledentistry are patient compliance and satisfaction regarding the dentist's physical presence, a low population education level, and a lack of technological infrastructure. A statistically significant relationship was found between teledentistry practice and age, marital status, working experience, and training regarding teledentistry. A positive correlation was found between knowledge of and attitude toward teledentistry. Conclusion: Dentists in Jakarta have a high understanding of, positive attitudes toward, and good practices for teledentistry. Factors related to teledentistry practice were age, marital status, work experience, and training experience in the last two years.

Maternal Education Level as a Risk Factor for Early Childhood Caries / Maternal Education Level as a Risk Factor for Early Childhood Caries

ABSTRACT Objective: To determine the prevalence and risk factors associated with dental caries in Brazilian preschool children aged 2 to 5 years. Material and Methods: The following independent variables were evaluated: dental caries, dental trauma, and malocclusions. Data were analyzed using the Mann-Whitney test and Poisson regression (p<0.05). Results: The prevalence of dental caries in preschoolers was 55.1%. Child age, family income, maternal education, and tooth color alteration from trauma were significantly associated with dental caries (p<0.05). The final adjusted multivariate model showed a significant association between maternal education and dental caries; children of mothers with low education were 1.89 times more likely to have caries (PR: 1.89; 95%CI: 1.40-2.55) (p<0.01). Children with tooth color change from trauma were more likely to have dental caries (PR: 1.69; 95%CI: 1.22-2.34 (p=0.001). Conclusion: Child age, family income, maternal education, tooth discoloration and discoloration color are risk factors for the development of caries disease in children. Also, children of mothers with low educational levels have a higher chance of developing dental caries.

Telemonitoramento dos idosos de natal-rn na atenção primária durante a pandemia da covid-19: estudo longitudinal descritivo / Telemonitoring of elderly people from Natal-RN in Primary Care during the COVID-19 pandemic: descriptive longitudinal study / Telemonitoreo de ancianos de Natal-RN en Atención Primaria durante la pandemia de COVID-19: estudio longitudinal descriptivo

Introdução:Atelessaúdese popularizou como uma importante ferramenta na avaliação remota e de prestação de cuidados em saúde.Uma de suas vertentes consiste no monitoramento remoto, também chamado de telemonitoramento ou televigilância,que se difundiu, sobretudo para grupos populacionais vulneráveis, como a população idosa, em especial durante a pandemia da COVID-19. Objetivo:Conhecer o perfil sociodemográfico, o estado de saúde e os comportamentos relacionados à saúde dos idosos monitorados durante a pandemia da COVID-19 no município de Natal-Rio Grande do Norte.Metodologia:Estudo longitudinal descritivo, com três momentos de observação. A amostra foi composta por idosos adscritos a 22 unidades de saúde de Natal, acompanhados por meio de ligações telefônicas, de agostode 2020 a julho de 2021. A análise dos dados foi realizada através do software Epi Info™, versão 7.2.4, a partir de frequências absolutas e relativas, medidas de tendência central e dispersão (média e desvio padrão).Resultados:Participaram do estudo 1.348 idosos. Amaioria era do sexo feminino (63,7%), com média de 70 anos, morava acompanhada(81,8%), esteve assintomática (77,0%), possuía comorbidades (81,5%) e dependia de medicações de uso contínuo (81,9%). Os sintomáticos diminuíram ao longo dos três momentos avaliados e menos de 1% evoluiu ao óbito.Conclusões:O telemonitoramentocontribuiu com a longitudinalidade do cuidado, proporcionando a busca ativa contínua de idosos sintomáticos e fortalecendo as atividades das Unidades de Saúde (AU).

Introduction:Telehealth has become popular as an important tool in the remote assessment and provision of health care. One ofits aspects is remote monitoring, also called telemonitoring or telesurveillance, which has become widespread, especially for vulnerable population groups, such as the elderly population, especially during the COVID-19 pandemic.Objective:To know the sociodemographic profile, health status and health-related behaviors of the elderly monitored during the COVID-19 pandemic in the Natal-Rio Grande do Norte. city.Methodology:Descriptive longitudinal study, with three moments of observation. The sample consisted of elderly people enrolled at 22 health units in Natal, followed up through telephone calls, from August 2020 to July 2021. Data analysis was performed using the Epi Info™ software, version 7.2.4, based on absolute and relative frequencies, measures of central tendency and dispersion (mean and standard deviation).Results:1,348 elderly people participated in the study. Most were female (63.7%), aged 70 years on average, lived with someone (81.8%), were asymptomatic (77.0%), had comorbidities(81.5%) and depended on continuous use (81.9%). Symptomatic cases decreased over the three evaluated moments and less than 1% evolved to death.Conclusions:Telemonitoring contributed to the longitudinality of care, providing continuous active search for symptomatic elderly and strengthening the activities of Health Units (AU).

Introducción: La telesalud se ha vuelto popular como una herramienta importante en la evaluacióny provisión remota de atención médica. Una de sus vertientes es el monitoreo remoto, también llamado telemonitoreo o televigilancia, que se ha generalizado, especialmente para grupos de población vulnerable, como la población de la tercera edad, especialmente durante la pandemia del COVID-19. Objetivo: Conocer el perfil sociodemográfico, el estado de salud y los comportamientos relacionados con la salud de los ancianos acompañados durante la pandemia de COVID-19 en la ciudad de Natal-Rio Grande do Norte.. Metodología: Estudio longitudinal descriptivo, con tres momentos de observación. La muestra estuvo compuesta por ancianos matriculados en 22 unidades de salud de Natal, seguidos a través de llamadas telefónicas, de agosto de 2020 a julio de 2021. El análisis de los datos se realizó mediante el software Epi Info™, versión 7.2.4, con base en valores absolutos y relativos. frecuencias, medidas de tendencia central y dispersión (media y desviación estándar). Resultados: Participaron del estudio 1.348 ancianos. La mayoría eran del sexo femenino (63,7%), con edad media de 70 años, vivían con alguien (81,8%), estaban asintomáticos (77,0%), tenían comorbilidades (81,5%) y dependían del uso continuo (81,9%). Los casos sintomáticos disminuyeron en los tres momentos evaluados y menos del 1% evolucionó a muerte.Conclusiones: La televigilancia contribuyó para la longitudinalidad de la atención, proporcionando búsqueda activa continua de ancianos sintomáticos y fortaleciendo las actividades de las Unidades de Salud (AU).

Análise da imunoexpressão de BAX e MMP-9 no líquen plano oral / Analysis of BAX and MMP-9 immunoexpression in oral lichen planus

Introdução: O líquen plano oral é uma doença crônica imunologicamente mediada relativamente comum, que acomete a mucosa oral. Clinicamente, o LPO é classificado em seis padrões bem identificados: placa, reticular, bolhoso, atrófico, papular e erosivo.Sendo os mais comuns oos tipos reticulares e erosivos. A ativação dos linfócitos TCD4+ no LPO, pode induzir os ceratinócitos ao processo de apoptose através da respostaimunológica citotóxica. A proteína Bax desempenha uma função relevante para o processo apoptótico. Deste modo, a presente pesquisa consistiu em um estudo transversal retrospectivo, descritivo, quantitativo e comparativo. Objetivo: Avaliar a expressão imuno-histoquímica das proteínas MMP9 e Bax no LPO. Método: Foram utilizados 43 casos de LPO para análise da imunoexpressão de Bax e MMP-9. Os resultados foram analisados através dos testes estatísticos apropriados e serão considerados significativos, valores onde p<0,05. Resultado: A imunoexpressão de MMP9 foi significativamente maior nos ceratinócitos e quando analisados os subtipos de líquen plano oral, não foram observados diferenças estatísticas entre os tipos reticulares e erosivos para as proteínas analisadas. Conclusões: Com essas observações, infere-se que a alteração na expressão das proteínas estudadas sugere um distúrbio nos mecanismos apoptóticos, os quais estão associados às lesões de LPO, e podemos concluir também que as imunoexpressões dessas proteínas não apresentaram diferença, quando relacionada ao tipo clínico reticular ou erosivo. Com esse resultado pode-se contribuir para um maior entendimento sobre os possíveis mecanismos celulares envolvidos na etiopatogenia dessa lesão (AU).

Background: Oral lichen planus is a relatively common immune-mediated chronic disease that affects the oral mucosa. Clinically, OLP is classified into six well-identified patterns: plaque, reticular, bullous, atrophic, papular, and erosive. The most common being the reticular and erosive types. The activation of TCD4+ lymphocytes in the OLP can induce keratinocytes to the process of apoptosis through the cytotoxic immune response. Thus, the present research consisted of a retrospective, descriptive, quantitative and comparative crosssectional study. Objective: to evaluate the immunohistochemical expression of MMP-9 and Bax proteins in OLP. Methods: We used 20 cases of Inflammatory Fibrous Hyperplasia as control. The results were analyzed through the appropriate statistical tests and will be considered significant, values where p<0.05. Results: The immunoexpression of MMP-9 was significantly higher in keratinocytes and when the subtypes of oral lichen planus were analyzed, no statistical differences were observed between the reticular and erosive types for the proteins analyzed. Conclusions: With these observations, it is inferred that the alteration in the expression of the studied proteins suggests a disturbance in the proliferative and apoptotic mechanisms, which are associated with a pathological behavior of the oral mucosa, and consequently with a repercussion on the lesions of OLP, and we can also conclude that the immunoexpression of these proteins had no difference, when related to the reticular or erosive clinical type. This research aims to contribute to a greater understanding of the possible cellular mechanisms involved in the etiopathogenesis of this lesion, thus enabling the understanding of the clinical aspects of the pathology (AU).

COVID-19 Related Knowledge, Attitude, Practice Evaluation among Indian Dental Patients: A Cross-Sectional Survey

ABSTRACT Objective: To assess the awareness about COVID-19 among dental patients and determine the association between COVID-19 awareness and perceived stress among dental patients. Material and Methods: A selfadministered knowledge, attitude and practices (KAP) questionnaire and perceived stress scale (PSS) were employed to assess the knowledge and attitude among dental patients visiting the outpatient department. A p-value of ≤ 0.05 was considered statistically significant. An independent t-test was used to compare the KAP and PSS scores based on age, gender, occupation and the responses to unscorable questions. Spearman's correlation was employed to assess the association between KAP and PSS scores. Results: The older participants (mean KAP score = 16±2.2), the participants who answered that the Coronavirus outbreak changed their daily routine (mean KAP score = 15.8±2.2) and the participants who answered that they changed their plans due to the Coronavirus outbreak (mean KAP score = 16±2.14) had significantly higher KAP scores when compared to their respective counterparts. The Spearman's correlation value of -0.45 suggested a moderate negative association between the KAP and PSS scores, but this correlation was not statistically significant. Conclusion: The participants had a moderate to good level of knowledge related to COVID-19 and were positive in their approach and outlook on overcoming the pandemic.

Factors Associated with the Intake of Ultra-Processed Cariogenic Foods by Preschoolers During the COVID-19 Pandemic

ABSTRACT Objective: To evaluate the characteristics and factors associated with the intake of ultra-processed cariogenic foods (UFC) by preschoolers during the COVID-19 pandemic. Material and Methods: This is a cross-sectional study involving parents of 672 children from two to five years old enrolled at public schools in Curitiba, Brazil. Parents answered a questionnaire about socioeconomic and demographic data, their behavior regarding the dietary education of their children (Parent Mealtime Action Scale - translated and validated for use in Brazil), and children's food intake (qualitative food frequency questionnaire - list of foods based on a report from the Pan American Health Organization). The data were analyzed using Poisson regression analysis (α=0.05). Results: About 43% of parents/guardians reported changes in their children's diet during the pandemic, being that diet got worse and better in 19% and 24% of the cases, respectively. The ultra-processed cariogenic foods with the highest daily intake frequencies were sweetened juices/sweetened drinks (0.52), followed by cookies (0.37), and candies (0.35). Parents with a lower level of education reported a daily frequency of UCF intake 1.36 times higher (PR=1.359; CI 95%: 1.106-1.669) in their children compared to those with a higher level of education. On the other hand, parents'/guardians' report of higher intake and greater offer of fruits and vegetables to children was associated with low UCF intake (PR=0.716; CI 95%: 0.592-0.866). Conclusion: The lower level of formal education of parents/guardians and lower availability of fruits and vegetables were related to higher consumption of ultra-processed cariogenic foods by children.

Caregivers' Sense of Coherence and Untreated Dental Caries in Children and Adolescents: A Cross-Sectional Study

ABSTRACT Objective: To associate caregivers' sense of coherence (SOC) and untreated caries with oral health status in children covered by a dental public health system. Material and Methods: A convenience sample of caregivers/children in Angra dos Reis, Brazil, was surveyed. Caregivers' SOC was evaluated using the 13-question version questionnaire. For both caregivers/children, sociodemographic information was acquired and caries experience was evaluated by DMFT/dmft and PUFA/pufa index. Statistical associations between children's untreated carious teeth and interest variables from the caregivers were evaluated by binary logistic regression assessed by generalized linear modeling. Results: A total of 233 pairs of caregivers-children were included. Children's untreated permanent and primary carious teeth represented 34.8% and 62.2% of the caries experience, respectively and at least one PUFA/pufa scored tooth was detected in 22.7% of them. Caregivers' DMFT was 13.5±7.0, while 33.6% scored on PUFA. A total of 62.1% of them presented untreated carious lesions. Results from the univariate model, correlating children's untreated caries and caregivers' attributes showed a statistical significance for SOC values (p<0.015), untreated decayed teeth (p<0.035), self-perception of oral health (p<0.022) and oral impact on daily performance (p<0.010). The multivariate logistic first model kept the statistical significance only for the caregiver's untreated decayed teeth. Conclusion: Caregivers' SOC and untreated carious teeth could be used as indicators of dental treatment needs in their offspring.

Impact of Mobile Dental Clinics on the Quality of Life of Children

ABSTRACT Objective: To evaluate the impact of mobile dental clinics on the oral health-related quality of life (OHRQL) of children. Material and Methods: A longitudinal epidemiological study was conducted with participants from seven mobile dental clinics carried out between May 2019 and January 2020 by the NGO Missão Sorrisos. Parents and children who attended the program had their sociodemographic data collected. Both completed the Scale of Oral Health 5 (SOHO-5) self-reported questionnaire before treatment and again 30 days after treatment. Results: The improvement in the children's oral health after treatment at the mobile clinics is reflected in the pre-and post-treatment medians measured by the SOHO-5 total score from the children's own reports from the parents' reports. The procedures performed were effective in reducing pain and difficulties in eating, drinking, and sleeping. An improvement in the perception of the children's appearance and self-confidence was reported, both from the perspective of the parents/guardians and the children themselves. The chance of improvement in the perception of the children's oral health was greater for parents (OR=5.96; CI95%: 1.32-26.84) and children (OR=5.76; CI95%: 1.28-25.95) from families whose main caregiver was not professionally active at the time of the study. Conclusion: The mobile dental clinics had a positive impact on the OHRQL of children from the perspective of the participants of the study.

Effect of Quality of Dental Health Services to Patient Satisfaction in Indonesia 2019: Importance-Performance Analysis

ABSTRACT Objective: To determine the effect of the quality of dental health services based on dimensions of empathy and responsiveness to patient satisfaction in urban and rural areas in Bone District, Indonesia. Material and Methods: This survey used an analytical observational method with a pilot pathfinder survey design. The total of participants included in this survey was 442, with 223 in the urban area and 219 in the rural area. This survey was held on 25 February - 1 March 2019. The Mann-Whitney test was applied, adopting a significance level of 5%. Results: Empathy and responsive dimensions in urban (3.58 ± 0.94 and 3.50 ± 0.94) and rural (3.43 ± 0.99 and 3.63 ± 0.86) areas were in the moderate category. The administration staff was able to complete administrative procedures, and the Dental assistant responded promptly to the patient's request and needs. Conclusion: The quality of empathy and responsiveness dimensions in dental treatment fall in the moderate category for urban and rural areas; this means that empathy and responsiveness dimensions in dental service in hospital/public health need to be improved.

Prevalence of Dental Caries in 5-Year-Old Children in a Northeast Brazilian Capital

ABSTRACT Objective: To evaluate the prevalence of dental caries in children aged five years in a Northeast Brazilian Capital (Fortaleza, CE) and its association with sociodemographic conditions, presence of malocclusion, and gingival bleeding. Material and Methods: This is a quantitative, descriptive, observational, and cross-sectional study carried out in a representative sample of 3,582 children aged five years in the city of Fortaleza-CE. Data was collected in public and private schools distributed in the city's Regional Health Coordination (CORES). Each of the six CORES worked with five field teams, participating in inter-examiner training and calibration with a final KAPPA coefficient of 0.87. Data were collected using a clinical form adapted from the SB Brasil 2010 questionnaire on sociodemographic conditions. The indices of dmft, need for treatment, malocclusion, and gingival bleeding were used for oral health conditions. The data were submitted to Pearson's Chi-square or Fisher's Exact tests, and the variables that show values of p<0.05 were submitted to a multinomial logistic regression model (forward stepwise model). Results: 57.1% of children were caries-free, and the mean dmft-d was 1.65 (1.65±2.65). CORES I and VI were the ones that presented the most significant association with caries attacks in all primary molars. The highest percentage of caries in the 2nd upper molar (60.6%), 1st lower molar (59%), and 2nd lower molar (58.8%) were found in children with normal occlusion. There was a significant association between gingival bleeding and caries in all molars and the need for treatment. Conclusion: These results allow us to observe that the prevalence of dental caries in 5-year-old children in Fortaleza is low, although with a tendency to increase.

Fatores associados ao interesse de usuários de prótese total mandibular em terapia implantossuportada e impacto da reabilitação com sobredentadura com implante único / Factors associated with the interest of users of complete mandibular dentures in implant-supported therapy and the impact of rehabilitation with single-implant overdentures

Este estudo objetivou identificar os fatores que influenciam na decisão de substituir uma prótese total convencional mandibular pelo tratamento com implantes dentários, assim como avaliar a satisfação, qualidade de vida e performance mastigatória (PM) após reabilitação com sobredentaduras com implante único (SIU) em pacientes adaptados e não-adaptados a prótese total convencional (PT) mandibular. Inicialmente, foi realizado um estudo transversal com 117 usuários de PTs bimaxilares, avaliados quanto aos desfechos relacionados à prótese (número de PTs mandibulares usadas previamente, tempo de uso e profissional que confeccionou a prótese atual, e quanto ao uso regular ou não da prótese mandibular) e aspectos centrados no paciente (período de edentulismo mandibular, altura óssea mandibular e interesse do paciente em se submeter a terapia com implantes). Do total da amostra, 78 pacientes manifestaram interesse na terapia implantossuportada, e desses apenas 22 foram selecionados e reabilitados com novas PTs bimaxilares. Após critérios de elegibilidade, foram alocados após pareamento em 2 grupos: adaptados à prótese mandibular (Grupo PTA - "adaptados à PT mandibular", n:10) e não adaptados (Grupo PTN - "não adaptados à PT mandibular", n:12). Em cada paciente, foi instalado um único implante na linha média e após o período de osseointegração as próteses mandibulares foram convertidas em sobredentaduras. O desempenho mastigatório foi avaliado pelo método das tamises, a altura óssea mandibular por medição em radiografia panorâmica, satisfação por escala quantitativa com questionário validado e o impacto da saúde oral na qualidade de vida pelo questionário OHIP-Edent-19. O teste Qui-quadrado foi utilizado para análise dos dados e as razões de prevalência ajustadas por meio da regressão multivariada de Poisson no primeiro estudo; para o segundo, a análise estatística em cada grupo e entre grupos foi baseada nos testes não-paramétricos de Wilcoxon e Mann-Whitney. O intervalo de confiança para os testes foi de 95%. A amostra foi predominantemente do sexo feminino, com 66,7% (n=78) dos participantes interessados em PT mandibular implantossuportada e com idade média de 65,68±6,38 anos. A PM não influenciou a escolha pela reabilitação com implantes. A preferência pela PT implantossuportada mandibular foi associada significativamente com maior experiência prévia com PT mandibular (p=0,021) e à insatisfação quanto à retenção (p=0,005). Após a intervenção com implante, todos os pacientes não adaptados passaram a condição de adaptados. Não houve diferença entre PTA e PTN para OHIP-Edent (p=0,276) e PM (p=0,222), a satisfação foi significativa apenas para o critério "conforto em arco inferior" (p=0,043). Para comparações pré e pós-tratamento com sobredentadura, a mediana do OHIP-Edent total diminuiu significativamente em ambos os grupos. Na comparação intragrupo, essa redução foi significativa em PTA apenas para a "limitação funcional" (p=0,026), e em PTN em quase todos os domínios, exceto "disfunção social" e "incapacidade" (p>0,05). Houve aumento estatisticamente significativo para a satisfação geral de 75,41 para 90,25 (p=0,012) em PTN e de 76,10 para 90,50 (p=0,007) em PTA. Os parâmetros "mastigação", "adaptação", "retenção" e "conforto" em arco inferior foram diferentes com significância em ambos os grupos, e "gustação", "fonação" e "dor" em arco inferior foram significativos apenas para o grupo não adaptado (p<0,05). Houve diferença significativa para PM em PTN (p=0,002) e PTA (p=0,047) ao se comparar tipo de reabilitação. Não houve correlação entre PM e OHIP antes e após a reabilitação (p>0,05). Pode-se concluir que a experiência prévia com PT mandibulares convencionais e a insatisfação com a retenção dessas, influenciam o interesse pela reabilitação com sobredentadura implantossuportada mandibular, assim como, a reabilitação com sobredentadura sobre implante único apresenta-se como alternativa aos pacientes não adaptados a PT convencional mandibular, auxiliando na aceitação do uso da prótese mandibular, assim como, comprovando efeito positivo na satisfação, qualidade de vida e performance mastigatória (AU).

This study aimed to identify the factors that influence the decision to replace a conventional mandibular complete denture by treatment with dental implants, as well as to evaluate satisfaction, quality of life and masticatory performance (MP) after rehabilitation with overdentures with a single implant in patients adapted and not adapted to conventional mandibular complete denture (CD). Initially, a cross-sectional study was carried out with 117 users of bimaxillaries CDs, who were evaluated for outcomes related to the denture (number of previous mandibular CDs, time of use and professional who made the current denture, and regarding regular use of the mandibular denture) and aspects centered patients (period of mandibular edentulism, mandibular bone height and the patient's willingness to undergo implant therapy). From the total sample, 78 patients expressed interest in implant-supported therapy, and of these, only 22 were selected and rehabilitated with new bimaxillary CDs. After eligibility criteria, they were allocated after pairing into 2 groups: adapted to mandibular prosthesis (PTA Group - "adapted to mandibular CD", n:10) and non-adapted (PTN Group - " not adapted to mandibular CD", n:12). In each patient, a single implant was installed in the midline and after the osseointegration period, the mandibular prostheses were converted into overdentures. Masticatory performance was evaluated by the sieve method, mandibular bone height by paranoid radiographic measurement, satisfaction by quantitative scale with a validated questionnaire and the impact of oral health on quality of life by the OHIP-Edent-19 questionnaire. The chi-square test was used for data analysis and the prevalence ratios adjusted by means of multivariate Poisson regression in the first study; on the second, the statistical analysis in each group and between groups was based on the non-parametric-Wilcoxon and Mann-Whitney tests. The confidence interval for the tests was 95%. The sample was composed predominantly by women, with 66.7% (n=78) of the participants interested in implantsupported mandibular CD, with mean age 65.68±6.38 years. MP was not influenced by the choice by dental implants rehabilitation. Preference for mandibular implant-supported CD was significantly associated with longer previous experience in mandibular CD (p=0.021) and with dissatisfaction regarding retention (p=0.005). After the implant intervention, all non-adapted patients became adapted. There was no difference between PTA and PTN for OHIP-Edent (p=0.276) and MP (p=0.222), satisfaction was significant only for the criteria "comfort in the lower arch" (p=0.043). For pre- and post-treatment comparisons with overdentures, the median total OHIP-Edent decreased significantly in both groups. In the intragroup comparison, this reduction was significant in PTA only for "functional limitation" (p=0.026), and in PTN in almost all domains, except "social dysfunction" and "disability" (p>0.05). There was a statistically significant increase in overall satisfaction from 75.41 to 90.25 (p=0.012) in PTN and from 76.10 to 90.50 (p=0.007) in PTA. The parameters "chewing", "adaptation", "retention" and "comfort" in the lower arch were significantly different in both groups, and "gustation", "phonation" and "pain" in the lower arch were significant only for the group not adapted (p<0.05). There was a significant difference for MP in PTN (p=0.002) and PTA (p=0.047) when comparing the type of rehabilitation. There was no correlation between MP and OHIP before and after rehabilitation (p>0.05). It can be concluded that the previous experience with conventional mandibular CD and the dissatisfaction with the retention of these, influence the rehabilitation with mandibular implant-supported overdenture, as well as the rehabilitation with overdenture on single implant presents itself as an alternative for patients not adapted to mandibular conventional CD, helping to accept the use of mandibular prosthesis, as well as proving a positive effect on satisfaction, quality of life and masticatory performance (AU).

Efeito do probiótico lactobacilus casei adjunto a terapia periodontal em modelo experimental de periodontite: uma relação entre periodonto e intestino / Effect of the probiotic lactobacilus casei as an adjunct to periodontal therapy in an experimental model of periodontitis: a relationship between periodontium and intestine

A periodontite é uma condição crônica inflamatória que pode influenciar a microbiota intestinal. O tratamento padrão ouro para a periodontite inclui a raspagem e o alisamento corono-radicular (RACR), porém em casos complexos pode-se utilizar terapias adjuvantes, como os probióticos. A utilização deste tratamento adjuvante poderá contribuir para a melhoria da condição periodontal e a simbiose intestinal. O objetivo deste estudo foi avaliar o efeito na inflamação periodontal e intestinal da utilização do Lactobacillus casei (LC) adjunto a RACR em camundongos Balb/c com periodontite, induzida por ligadura. Este estudo é um ensaio pré-clínico, in vivo, randomizado, cego e controlado por placebo, constituído por 36 camundongos Balb/c machos. Os animais foram submetidos a indução da periodontite por colocação de ligadura com fio de seda 4.0 ao redor do segundo molar superior direito, sendo divididos em 4 grupos,o grupo controle: Sem Periodontite e sem RACR (n=8); Grupo Ligadura: Com Periodondite e sem RACR (n=10); Grupo raspagem: Com Periodontite e com RACR (n=10) ; Grupo raspagem + L.casei (n=8): Com Periodontite e com RACR + administração de LC, por gavagem, durante 30 dias. Foram realizadas análises de citocinas pelo método ELISA no tecido gengival (IL-6), intestinal (IL-1ß, IL-6 e IL-10) e sanguíneo (IL-1ß e IL-6), além de análises bioquímicas (TGO, TGP, ureia e creatinina) e contagem diferencial de leucócitos do sangue. Foram coletados fragmentos do intestino grosso desses animais e analisados quanto a biomarcadores do estresse oxidativo (SOD, GSH e MDA), atividade da acetilcolinesterase (AChE) e foi realizada contagem da população de Bactérias Produtoras de Ácido Láctico das fezes dos animais. A utilização do LC adjunto a RACR resultou em uma redução na expressão da IL-6 no tecido gengival de camundogos com Periodontite (p < 0,05). Para as inteleucinas séricas (IL-1ß e IL-6), não houve diferenças entre os grupos (p > 0,05). Já para as citocinas intestinais houve uma redução na expressão de IL-10 (p < 0,05), para os grupos em que foi induzida a Periodontite. Com relação ao estresse oxidativo intestinal os animais do Grupo raspagem e raspagem + LC tiveram uma redução dos níveis de MDA (p < 0,05), para a SOD e o GSH, não houve diferenças significativas entre os 4 grupos pesquisados (p < 0,05). Conclui-se que o uso de LC adjunto a RACR em camungongos com periodontite induzida por ligadura pode reduzir a liberação de IL-6 no tecido gengival. Com relação aos efeitos intestinais foi observada a modulação da resposta inflamatória, com a redução de MDA, nos animais que receberam o tratamento periodontal. E a redução da expressão da IL-10, nos animais com Periodontite (AU).

Periodontitis is a chronic inflammatory condition that can influence the gut microbiota. The gold standard treatment for periodontitis includes scaling and crown-root planing, but in complex cases adjuvant therapies such as probiotics can be used. The use of this adjuvant treatment may contribute to the improvement of periodontal condition and intestinal symbiosis. The aim of this study was to evaluate the effect on periodontal and intestinal inflammation of the use of Lactobacillus casei (LC) adjunct to scaling and root planing (RACR) in Balb/c mice with ligature-induced periodontitis. This study is a preclinical, in vivo, randomized, blinded, placebo-controlled trial consisting of 40 male Balb/c mice. The animals were submitted to periodontitis induction by placing a 4.0 silk suture ligature around the upper right second molar. The sample was divided into 4 groups, each with 10 animals: Group I: Without Periodontitis and without RACR; Group II: With Periodontitis and without RACR; Group III: With Periodontitis and with RACR; Group IV: With Periodontitis and with RACR + gavage of LC, for 30 days. Cytokine analyzes were performed by the ELISA method in gingival tissue (IL-6), intestinal tissue (IL-1ß, IL-6 and IL-10) and blood (IL-1ß and IL-6), the blood was also subjected to analysis biochemical (TGO, TGP, urea and creatinine) and differential leukocyte count. Fragments of the large intestine of these animals were collected and analyzed for biomarkers of oxidative stress (SOD, GSH and MDA), acetylcholinesterase (AChE) activity, and the population of Lactic Acid-Producing Bacteria in the animals' feces was counted. The use of LC adjunct to RACR resulted in a reduction in the expression of IL-6 in the gingival tissue of mice with Periodontitis (p < 0.05), for the blood inteleukins (IL-1ß and IL-6), there were no differences between the groups (p > 0.05). As for intestinal cytokines, there was a reduction in the expression of IL-10 (p < 0.05), for the groups that presented Periodontitis. Regarding intestinal oxidative stress, the animals in Groups III and IV had a reduction in MDA levels (p < 0.05), for SOD and GSH, there were no significant differences between the 4 groups studied (p < 0.05 ). It is concluded that the use of LC adjunct to RACR in mice with ligation-induced periodontitis can reduce the release of IL-6 in the gingival tissue. Regarding the intestinal effects, two effects were found: The first related to the modulation of the inflammatory response, with the reduction of MDA, in the animals that received periodontal treatment. And the second related to a pro-inflammatory effect, with the reduction of IL-10 expression (AU).

Influência de diferentes intermediários protéticos na satisfação e qualidade de vida de pacientes reabilitados com próteses unitárias livres de metal sobre implantes: um ensaio clínico / Influence of different prosthetic intermediates on the satisfaction and quality of life of patients rehabilitated with metal-free single prostheses on implants: a clinical trial

Introdução: Intermediários protéticos de diferentes formas, materiais ou métodos de confecção utilizados em tratamento com coroas sobre implante podem gerar impacto sobre o resultado final da reabilitação. Objetivo: Avaliar a interferência de dois intermediáros protéticos: (1) pré-fabricados em titânio (GT) ou (2) personalizados em zircônia (GZ) sobre a satisfação e qualidade de vida dos pacientes reabilitados por meio de coroas livres de metal sobre implantes unitários instalados na região do sorriso. Material e métodos: Um ensaio clínico não randomizado-controlado e cego foi realizado. Todos os pacientes passaram por uma etapa de coroa provisória antes da instalação da coroa final. A satisfação foi avaliada por meio da escala visual analógica ­ EVA e a qualidade de vida por meio do Oral Impacts on Daily Performances (OIDP) e do Oral Health Impact Profile (OHIP-14). As avaliações aconteceram em três tempos, baseline (T0), sete dias após a instalação da coroa provisória (T1) e sete dias após a instalação da coroa final (T2). Os dados foram avaliados por meio do teste de Mann-Whitney, para as análises intergrupos, e do teste de Wilcoxon para as avaliações intragrupos. O teste de correlação de Spearman foi realizada para avaliação dos fatores que interferiram na estética final. Resultados: Vinte e quatro pacientes foram incluídos, a média de idade foi de 41,88 anos. Foram instalados 26 implantes, 14 no GT e 12 no GZ, a região anterior teve 20 implantes instalados e a região posterior teve 06 implantes. Houve uma melhora significativa da satisfação, independentemente do grupo, entre T0 e T1 (p=0,013) e entre T1 e T2 (p<0,001). Considerando a amostra como um todo, as dimensões do OHIP-14 foram influenciadas positivamente entre T0 e T1 (p<0,001) e T0 e T2 (p<0,001). E as performances do OIDP também melhoraram entre T0 e T1 (p=0,007), T1 e T2 (p=0,007) e T0 e T2 (p<0,001). Conclusão: Os diferentes intermediários protéticos testados nesta pesquisa parecem não interferir nas variáveis estudadas e a instalação de coroa livre de metal sobre implante parece desempenhar um papel importante na melhoria dos níveis de satisfação e qualidade de vida dos pacientes (AU).

Introduction: Prosthetic abutment of different shapes, materials or fabrication methods used in oral rehabilitation can play a role in the success of the rehabilitation. Objective: To evaluate the influence of two different abtments: (1) prefabricated titanium (GT) or (2) customized zirconia (GZ) in the quality of life, and patients' satisfaction wearing metal-free crowns on single implants placed in the smile region. Material and methods: A non-randomized-controlled-blinded clinical trial was performed. All patients received a temporary restoration before the final metal-free crown. The patient's satisfaction was assessed by a Visual Analog Scale (VAS), and quality of life by the Oral Impacts on Daily Performances (OIDP) and the Oral Health Impact Profile (OHIP-14). The follow-up was performed at baseline (T0), seven days after provisional crown placement (T1) and seven days after the final crown (T2). Data were assessed using the MannWhitney test for intergroup analyses, and the Wilcoxon test for intragroup assessments. The Spearman correlation test was performed to evaluate the factors that can influence the final esthetics. Results: Twenty-four patients were included; the mean age was 41.88 years. Twenty-six implants were placed, fourteen in GT and twelve in GZ. Twenty implants were placed in the anterior region and 06 in the posterior. A significant improvement was noticed in the patient's satisfaction, regardless of the group, between T0 and T1 (p=0.013) and T1 and T2 (p<0.001). Considering the whole sample, the dimensions of the OHIP14 were positively influenced between T0 and T1 (p<0.001) and T0 and T2 (p<0.001). The performances of the OIDP also improved between T0 and T1 (p=0.007) as well as between T1 and T2 (p=0.007) and between T0 and T2 (p<0.001). Conclusion: The prosthetic abutments tested in this study do not seem to play a role in the variables studied and the metal-free crown placement seems to play an important role in improving the levels of patient's satisfaction and quality of life (AU).

Assessment and associations between quality of life and risk of suicide / Avaliações e associações entre qualidade de vida e risco de suicídio / Evaluaciones y asociaciones entre calidad de vida y riesgo de suicidio

This study aimed to verify the extent to which the levels of quality of life are related to and influence suicide risk indices. A descriptive, correlational, quantitative research was performed. A non-probabilistic sample was used which was composed of 11,863 Brazilian participants all over 18 years old, who responded to the Risk Assessment Suicide Scale (RASS) and the World Health Organization Quality of Life BREF Instrument (WHOQOL-BREF), and whose data were analyzed by using descriptive statistics, the Spearman correlation and multiple linear regression using the Statistical Package for Social Sciences (SPSS). The results identify negative, moderate and significant correlations between the constructs, and that the domains of quality of life influence 47.8% of the variations in the suicide risk indices, confirming that the psychological domain is the most relevant within the explanatory model created. The conclusion is that there is a the need for public policies to improve the quality of life and prevent suicide (AU).

Este estudo objetivou verificar em que medida os níveis de qualidade de vida se relacionam e influenciam os índices de risco de suicídio. Realizou-se uma pesquisa descritiva, correlacional, de abordagem quantitativa. Contou-se com uma amostra não probabilística composta por 11.863 participantes - brasileiros e maiores de 18 anos, que responderam à Risk Assessment Suicide Scale (RASS) e ao Word Health Organization Quality of Life Instrument Bref(WHOQOL-bref), cujos dados foram analisados por meio de estatística descritiva, correlação de Spearman e regressão linear multivariada pormeio do Statistical Package for Social Science (SPSS). Os resultados identificam correlações negativas, moderadas e significativas entre os construtos; e que os domínios de qualidade de vida influenciam em 47,8% as variações nos índices de risco de suicídio, constatando que o domínio psicológico é o mais relevante dentro do modelo explicativo criado. Conclui-se a necessidade de políticas públicas para melhoria da qualidade de vida e prevenção de suicídio (AU).

Este estudio objetivó comprobar hasta qué medida los niveles de calidad de vida se relacionan e influyen en las tasas de riesgo de suicidio. Se realizó una investigación descriptiva, correlacional y cuantitativa. Hubo una muestra no probabilística compuesta por 11.863 participantes brasileños mayores de 18 años, que respondieron a la Risk Assessment Suicide Scale (RASS) y al Word Health Organization Quality of Life Instrument Bref(WHOQOL-bref), cuyos datos fueron analizados mediante estadística descriptiva, correlación de Spearman y regresión lineal multivariante en Statistical Package for Social Science (SPSS). Los resultados identifican correlaciones negativas, moderadas y significativas entre los constructos; y que los dominios de calidad de vida influyen en un 47,8% en las variaciones de los índices de riesgo de suicidio, encontrando que el dominio psicológico es el más relevante dentro del modelo explicativo creado. Estos hallazgos revelan la necesidad de políticas públicas para mejorar la calidad de vida y prevenir el suicidio (AU).

Comparison of Oncostatin M in Patients with Chronic Periodontitis with and without Diabetes

Abstract Objective: To compare the Oncostatin M (OSM) concentrations in tissues of patients with chronic periodontitis with and without diabetes. Material and Methods: Sixty-four subjects visiting the dental outpatient department were categorized as "healthy" (Group 1), "periodontitis" (Group 2), and "diabetes with periodontitis" (Group 3) groups. The clinical oral examination included assessment of plaque, gingivitis, probing depth, clinical attachment level. Blood glucose was assessed for group 3 patients. OSM concentration in the tissues was assessed using ELISA in all groups. Results: The mean OSM was 0.02 ± 0.04 pg/mg in the healthy group, 0.12 ± 0.09 pg/mg in the chronic periodontitis group and 0.13 ± 0.10 pg/mg in the diabetes-periodontitis group. A significantly higher mean OSM was seen in Group 2 and Group 3 than Group 1. The amount of OSM positively correlated with probing depth and clinical attachment level. Conclusion: Periodontal disease causes a rise in Oncostatin M, independent of the diabetic status. Expression of OSM in the gingival tissues can serve as an inflammatory marker.

Does the Use of Surface Electromyography Could Improve Quality of Life among Patients Rehabilitated by Mandibular Overdentures on Different Attachments?

Abstract Objective: To analyze and compare changes of quality of life parameter among dental patients rehabilitated by the implant-supported overdentures with different attachment systems. Material and Methods: Forty-eight patients were recruited as a study cohort. The implant placement procedure was based on the results obtained by CBCT scanning and individualized surgical templates manufactured for correct implant placement. Each individual received two k3Pro Implants (Sure Type with 4.0 or 4.5 mm in diameter) at the intraforaminal area due to standard protocol of implantation provided by the manufacturer under local anesthesia. All patients were distributed between two groups based on the fact of using either Locator- or ball-attachments. Rank correlation was measured using Spearman correlation coefficient, while linear correlation was evaluated by Pearson correlation coefficient. Results: No statistically meaningful differences were noted regarding patients' distribution among groups considering age (p>0.05) and gender (p>0.05). Provided patient-level analysis demonstrated that increase of conventional full denture service time was positively correlated with escalation of OHIP-EDENT scores. The most prominent inter-correspondences were noted specifically between longevity of denture service and elevation of scores within "Functional limitation" (r=0.61; p<0.05), "Physical pain" (r=0.51; p<0.05) and "Physical disability" (r=0.57; p<0.05) subdomains. No statistically argumented regressions were noted between increase tendency of OHIP-EDENT scores and gender (p>0.05) or age (p>0.05) parameters. Conclusion: Significant improvements of quality of life measured with OHIP-EDENT were noted for both types of attachments compared to the pre-treatment situation independently of additionally provided surface electromyography-based alignment.

Knowledge of Dentists and Dental Undergraduates Related to COVID-19

Abstract Objective: To assess the knowledge of Brazilian dentists and final-year dental undergraduates concerning COVID-19. Material and Methods: We conducted a self-administered online questionnaire about the symptoms, incubation period, and transmission routes of COVID-19. In total, there were three questions addressing these topics and 15 correct answers, so each participant could score from 0 to 15 points. Besides that, data such as sex, age, education level, years of work experience and place of work were collected. All data were submitted to statistical analysis with a 5% significance level. Results: 476 participants were recruited. Regarding the respondents' perception of the most common symptoms of COVID-19, 99.4% responded fever, 95.2% cough, and 99.2% dyspnea. About the incubation period, 56.3% answered from 1 to 14 days. About the transmission routes, 98.3% recognized transmission through droplets, 80.3% through direct contact with infected persons, and 70.4% through indirect routes. The median knowledge score was 10 (4 - 14). Regarding the socio-demographic variables, participants aged 30 years or more had a higher score than those aged up to 29 years old (p=0.004). For education level, specialist dentists presented a higher score than undergraduates (p=0.006), general dentists (p=0.048) and Ph.D. (p=0.016). Participants with 15 years or more of work experience had a higher score than undergraduates (p=0.003). Concerning the workplace, participants working in the public sector had a higher score than those working in the private sector or universities (p=0.015). Conclusion: Participants recognized the main symptoms, incubation period, and transmission routes of the COVID-19 virus; however, the knowledge level of specialist dentists, older dentists, more experienced dentists, and dentists working in the public sector was higher than the other participants (AU).

Self-Declared Oral Health Conditions and Oral Health-Related Quality of Life of the Brazilian Homeless Population: A Cross-Sectional Study

Abstract Objective: To associate the impact of oral health-related quality of life with socio-demographic determinants, self-perception, oral health conditions and access to dental treatment among homeless adults. Material and Methods: A cross-sectional study was carried out in a medium-sized Brazilian city in Minas Gerais. Socio-demographic data on the use of dental services and access to hygiene supplies for oral health were collected. Clinical aspects were collected by clinical observation using the Community Oral Health Indicator and oral health-related quality of life using the Oral Health Impact Profile (OHIP-14). Medians and interquartile distance were compared using the Mann-Whitney and Kruskal-Wallis tests. Multiple linear regression included significant variables (p<0.05) of the bivariate analysis according to each OHIP-14 domain. Results: Women had the lowest scores for the physical and social disability domains. Oral health conditions were associated with physical and psychological limitations and disability. The presence of more teeth was associated with a negative impact on the functional and physical domains, and inflamed gums, lack of need for dental prosthesis, and use of private services were associated with a greater psychological disability. Conclusion: Oral health-related quality of life of the homeless population is affected, especially in the physical and psychological aspects. Equitable and inclusive health actions aimed at this population should include oral health (AU).

Psychological Factors in Temporomandibular Disorders Patients during COVID-19 Pandemic: A Cross-Sectional Study

Abstract Objective: To assess the influence of the COVID-19 pandemic on levels of anxiety, stress, and orofacial pain in individuals with and without TMD. Material and Methods: In this cross-sectional study, the participants were divided into two groups (n=10, each) experimental group (EG) TMD patients and control group (CG), without TMD. The presence of TMD was confirmed using the RDC/TMD. The Beck Anxiety Scale (BAI), Perceived Stress Scale (PSS), and Oral Analog Scale were used for assessment. The Beck Anxiety Scale is composed of 21 multiple-choice questions describing symptoms of anxiety, while the PSS is a measure that evaluates life situations as stressful. An Oral Analogue Scale was applied to each individual to quantify orofacial pain, with 0 (zero) without pain and 10 (ten) being the worst pain ever felt. The comparison between groups was performed using the Mann-Whitney test. Logistic regression was used to verify TMD dependence in relation to BAI and PSS-10. Quantitative variables were correlated with each other by Pearson's correlation coefficient. The level of significance considered for all tests was 5%. Results: The association was statistically significant (p≤0.05) for BAI and PSS with a TMD. Conclusion: Although the COVID 19 pandemic has had a psychological impact on the general population, patients with TMD have higher levels of anxiety and stress than the control group.

Sífilis gestacional e congênita: evolução e relação com estratégia saúde da família no sul e extremo sul baiano / Gestational and congenital syphilis: evolution and relationship with the family health strategy in Southern Bahia / Sífilis gestacional y congénita: evolución y relación con la estrategia de salud familiar sur/extremo sur de Bahia

Sífilis em gestantes e sífilis congênita representam importantes problemas de saúde pública no Brasil. Este estudo descreve a situação epidemiológica dos casos de sífilis em gestantes e sífilis congênita na região sul/extremo sul baiana e compara a evolução das taxas de detecção dessas doenças com a ampliação da cobertura da Estratégia Saúde da Família (ESF). Este é um estudo observacional e descritivo do período de 2009-2019. Foram analisados municípios agrupados em diferentes portes populacionais e casos analisados, segundo características maternas e assistenciais. Foram correlacionadas taxas de detecção e incidência com a cobertura da Estratégia Saúde da Família utilizando o coeficiente de correlação de postos de Spearman. Os dados foram coletados do Painel de Indicadores Epidemiológicos do Ministério da Saúde. O grupo com população inferior a 20 mil habitantes foi excluído pela precariedade de registros. Foram notificados um total de 3.028 casos de sífilis em gestantes e 1.454 de sífilis congênita, nos 22 municípios selecionados, que formaram três grupos. Todos os grupos apresentaram aumento nas taxas de detecção e incidência e, quando correlacionadas com a cobertura da Estratégia de Saúde da Família, mostraram resultados positivos, sendo os mais fortes para o de menor população. Em todos os grupos prevaleceram casos de mulheres jovens, com baixa escolaridade, em sua maioria negras e que realizaram pré-natal, mas com diagnóstico de sífilis primária no terceiro trimestre gestacional. O tratamento da gestante e do parceiro foi inadequado ou não realizado na maioria dos grupos. O desafio atual está em qualificar o pré-natal, controlar desigualdades e barrar a transmissão vertical.

Gestational and congenital syphilis is a serious public health issue in Brazil. This study describes the epidemiological situation of gestational and congenital syphilis cases in southern Bahia, comparing the evolution of the detection rates of these diseases with the expanded coverage of the Family Health Strategy (FHS). An observational and descriptive research was conducted for the period of 2009-2019, in municipalities grouped by population sizes, analyzing cases according to maternal and care characteristics. Detection and incidence rates were correlated with the Family Health Strategy coverage using Spearman's rank correlation coefficient. Data were collected from the Epidemiological Indicators Panel of the Ministry of Health. The group with a population of less than 20,000 inhabitants was excluded due to poor records. A total of 3028 cases of gestational syphilis and 1454 cases of congenital syphilis were reported in the 22 selected municipalities divided into three groups. All groups presented increased detection and incidence rates and showed positive correlations with the coverage of the Family Health Strategy, stronger for the smallest population. Results show a prevalence of cases involving mostly young black women, with low schooling level, who received prenatal care, with a diagnosis of primary syphilis in the third gestational trimester. Treatment of the pregnant woman and her partner was inadequate or not performed in most groups. The current challenge is to qualify prenatal care, control inequalities, and stop vertical transmission.

Gestational and congenital syphilis is a serious public health issue in Brazil. This study describes the epidemiological situation of gestational and congenital syphilis cases in southern Bahia, comparing the evolution of the detection rates of these diseases with the expanded coverage of the Family Health Strategy (FHS). An observational and descriptive research was conducted for the period of 2009-2019, in municipalities grouped by population sizes, analyzing cases according to maternal and care characteristics. Detection and incidence rates were correlated with the Family Health Strategy coverage using Spearman's rank correlation coefficient. Data were collected from the Epidemiological Indicators Panel of the Ministry of Health. The group with a population of less than 20,000 inhabitants was excluded due to poor records. A total of 3028 cases of gestational syphilis and 1454 cases of congenital syphilis were reported in the 22 selected municipalities divided into three groups. All groups presented increased detection and incidence rates and showed positive correlations with the coverage of the Family Health Strategy, stronger for the smallest population. Results show a prevalence of cases involving mostly young black women, with low schooling level, who received prenatal care, with a diagnosis of primary syphilis in the third gestational trimester. Treatment of the pregnant woman and her partner was inadequate or not performed in most groups. The current challenge is to qualify prenatal care, control inequalities, and stop vertical transmission.