Ancestry, health, and lived experiences of enslaved Africans in 18th century Charleston: An osteobiographical analysis.

OBJECTIVES: In 2013, the burials of 36 individuals of putative African ancestry were discovered during renovation of the Gaillard Center in downtown Charleston, South Carolina. The Charleston community facilitated a bioarchaeological and mitogenomic study to gain insights into the lives of these unknown persons, referred to as the Anson Street Ancestors, including their ancestry, health, and lived experiences in the 18th century. METHODS: Metric and morphological assessments of skeletal and dental characteristics were recorded, and enamel and cortical bone strontium stable isotope values generated. Whole mitochondrial genomes were sequenced and analyzed. RESULTS: Osteological analysis identified adults, both females and males, and subadults at the site, and estimated African ancestry for most individuals. Skeletal trauma and pathology were infrequent, but many individuals exhibited dental decay and abscesses. Strontium isotope data suggested these individuals mostly originated in Charleston or sub-Saharan Africa, with many being long-term residents of Charleston. Nearly all had mitochondrial lineages belonging to African haplogroups (L0-L3, H1cb1a), with two individuals sharing the same L3e2a haplotype, while one had a Native American A2 mtDNA. DISCUSSION: This study generated detailed osteobiographies of the Anson Street Ancestors, who were likely of enslaved status. Our results indicate that the Ancestors have diverse maternal African ancestries and are largely unrelated, with most being born locally. These details reveal the demographic impact of the trans-Atlantic slave trade. Our analysis further illuminates the lived experiences of individuals buried at Anson Street, and expands our understanding of 18th century African history in Charleston.

Oral microbial diversity in 18th century African individuals from South Carolina.

As part of the Anson Street African Burial Ground Project, we characterized the oral microbiomes of twelve 18th century African-descended individuals (Ancestors) from Charleston, South Carolina, USA, to study their oral health and diet. We found that their oral microbiome composition resembled that of other historic (18th-19th century) dental calculus samples but differed from that of modern samples, and was not influenced by indicators of oral health and wear observed in the dentition. Phylogenetic analysis of the oral bacteria, Tannerella forsythia and Pseudoramibacter alactolyticus, revealed varied patterns of lineage diversity and replacement in the Americas, with the Ancestors carrying strains similar to historic period Europeans and Africans. Functional profiling of metabolic pathways suggested that the Ancestors consumed a diet low in animal protein. Overall, our study reveals important insights into the oral microbial histories of African-descended individuals, particularly oral health and diet in colonial North American enslavement contexts.

Patterns of skeletal trauma in suicidal bridge jumpers: a retrospective study from the southeastern United States.

In the discovery of human remains from water environments, manner of death may not be immediately obvious to medicolegal investigators due to several factors, including lack of associated material evidence, nondescript contextual environment, or possible poor preservation of remains due to delayed recovery. The determination of patterns of skeletal trauma in suicidal bridge jumpers assists investigators in determining whether the manner of death was suicide versus non-suicide. This study reports on the patterns of skeletal trauma sustained in individuals who jumped from one of four large bridges in Charleston Harbor, South Carolina, and explores victim demographics, bridge height, position of the body upon impact, and velocity at impact on skeletal trauma for this suicide population. Data for all bridge jumpers were collected from coroner files spanning the years 1990-2011. Skeletal trauma is more heavily focused in the thorax/ribs (63%) and craniofacial (30%) regions. Fifty-six percent of jumpers sustained polytrauma. Comparative data on drowning victims, bodies recovered from boating/airplane accidents, and individuals who died by other suicidal means all show patterns of injury different than bridge jumpers.

Pattern of injury in child fatalities resulting from child abuse.

According to the US Department of Health and Human Services, in 2005, an estimated 1460 children died of maltreatment. The purpose of this study is to further examine the pattern of bony injuries in child maltreatment fatalities, with an emphasis on the prevalence of antemortem fractures and the presence of associated perimortem fractures. The sample was 162 male and female children. The majority of the data were collected from the case files of the NC Child Fatality Prevention Team at the Office of the Chief Medical Examiner in Chapel Hill, North Carolina (n=152) spanning from 2000 to 2005. An additional 10 cases from 2001 to 2006 were included from the Charleston County Coroner's Office, Charleston, SC. Six age categories were used in this study: 0-3 months, 4-6 months, 7-9 months, 10-16 months, 17 months to 2 years, and 2-6 years. Lesions were documented and categorized into four general body loci: craniofacial, thoraco/abdominal, appendicular, and multiple. The peak age categories of death were 0-3 months (25%) and 2-6 years (19%), with 50% of deaths occurring in infants 9 months old or younger. The body locus most frequently affected was craniofacial.